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使用自动化系统处理和冷保存细胞治疗产品的简化方法

Ye Li1, Hazel Y Stevens1, Srikanth Sivaraman1

  • 1Marcus Center for Therapeutic Cell Characterization and Manufacturing, Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, Georgia, USA.

Bio-protocol
|December 29, 2023
PubMed
概括

本研究提出了精简的良好实验室实践,用于处理和冷保存细胞疗法,确保生物制造和临床使用的高细胞活力. 该协议适用于附着介质细胞 (MSC) 和悬浮周围血液单核细胞 (PBMC).

关键词:
自动化系统自动化系统生物制造 生物制造 生物制造细胞疗法 细胞疗法结冷保存是一种冷保存.菲尼亚®填充和完成系统介质细胞层细胞是介质细胞层细胞.周围血液中的单核细胞.过程开发 过程开发

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Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
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