人体阴道微生物群的殖民是通过雌性性激素在小鼠模型中调节的
Nuzhat Rahman1,2, M Firoz Mian1,2, Aisha Nazli1,2
1Department of Medicine, McMaster University, Hamilton, ON, Canada.
Frontiers in cellular and infection microbiology
|December 29, 2023
概括
雌激素对于小鼠的阴道微生物群殖民至关重要,可能是通过糖原. 这突显了性激素和阴道健康之间的动态相互作用,影响了细菌多样性和殖民成功.
科学领域:
- 生殖生物学 生殖生物学
- 微生物组研究 微生物组研究
- 妇科 妇科 妇科 妇科
背景情况:
- 富含乳杆菌的阴道微生物群 (VMB) 是生殖健康的最佳选择.
- 细菌性阴道炎 (BV),以无氧VMB为特征,增加了对性传播感染和不良生殖结果的易感性.
- 目前的小鼠模型缺乏模仿eubiotic和dysbioticVMB的能力.
研究的目的:
- 开发小鼠模型,用于研究eubiotic和dysbiotic阴道微生物群.
- 研究性激素在VMB殖民和稳定中的作用.
- 探索潜在的机制,如糖原,调解激素-VMB相互作用.
主要方法:
- 在C57BL/6小鼠中开发了益生菌,益生菌和异生菌VMB模型,使用特定的Lactobacillus和Gardnerella vaginalis菌株.
- 通过卵巢切除 (OVX) 和17β-雌激醇或孕激素的使用来操纵内源性激素.
- 评估了细菌殖民,阴道糖原水平和MUC-1表达.
主要成果:
- 乳酸菌和G. vaginalis菌株在小鼠中显示过渡殖民,G. vaginalis从粘素存在中受益.
- 卵巢切除显著降低了VMB细菌负载,并削弱了外源细菌的殖民.
- 17β-雌激醇,但不是孕激素,恢复了内源性VMB并支持了殖民,与增加的阴道糖原水平相关.
结论:
- 在小鼠模型中,雌激素在促进阴道微生物群的殖民方面发挥着重要作用,可能是通过糖原介导的机制.
- 性激素与阴道微生物群发生动态相互作用,影响细菌多样性和殖民.
- 这些发现为开发针对VMB相关疾病的改进干预措施提供了基础.
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