促炎性细胞因子IL-6通过AKT/STAT3通路调节牛皮类角质细胞中的LMO4表达
Zhenzhen Tu1, Wei Wei2, Qiantong Xiang3
1Department of Immunology, School of Basic Medical Sciences, Anhui Medical University, Hefei, China.
Immunity, inflammation and disease
|December 29, 2023
概括
介质素-6 (IL-6) 通过激活AKT/STAT3通路来驱动牛皮皮肤细胞生长,从而导致高的LIM-only蛋白4 (LMO4) 表达和异常的角质细胞增殖.
科学领域:
- 皮肤病学 皮肤病学
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- 牛皮涉及表皮层增生和炎症.
- 只有LIM蛋白4 (LMO4) 在牛皮的皮肤中过度表达,并与角质细胞的增殖有关.
- 介质素-23 (IL-23) 通过AKT/STAT3信号诱导LMO4,但IL-6的作用尚不清楚.
研究的目的:
- 为了研究INTERLEUKIN-6 (IL-6) 在调节皮病中LIM-only蛋白4 (LMO4) 表达中的作用.
- 阐明IL-6影响LMO4表达和角质细胞行为的分子机制.
主要方法:
- 使用化学发光免疫测试在患者血中的量化IL-6水平.
- 通过imiquimod诱导建立了类似牛皮的小鼠模型.
- 在用IL-6刺激的培养皮细胞 (HaCaT) 中利用了西部斑点,免疫光,免疫组织化学和EDU测定.
主要成果:
- 与对照组相比,在牛皮患者中观察到IL-6水平升高.
- 高LMO4,IL-6,p-AKT和p-STAT3表达与牛皮病变相关 (人类和小鼠模型).
- IL-6激活了AKT/STAT3通路,增加了LMO4的表达,并促进了HaCaT细胞的增殖和分化.
结论:
- 通过AKT/STAT3信号通路,IL-6可通过牛皮类角质细胞上调LMO4的表达.
- IL-6有助于皮肤上异常的角质细胞的增殖和分化在牛皮.
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