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在每天的光过渡期间重编程翻译组,因为它受到细胞质甘甲基-3-酸盐脱酶GAPC1/C2的影响

Melanie Wegener¹, Marcus Persicke², Karl-Josef Dietz^1,2

¹Department of Biochemistry and Physiology of Plants, Faculty of Biology, Bielefeld University, Universitätsstr.25, D-33615, Bielefeld, Germany.

Journal of experimental botany

|December 29, 2023

概括

细胞质甘-3-酸脱酶 (GAPC1/2) 在Arabidopsis中调节光暗过渡期间的翻译体. 淘汰突变者在这些关键的每日切换点显示出显著的代谢和转化重编程.

科学领域:

植物分子生物学植物分子生物学
植物生理学植物生理学
代谢学代谢学代谢学

背景情况:

光暗转换对于植物代谢调节至关重要.
细胞质甘甲基-3-酸盐脱酶 (GAPC1/2) 已知在新陈代谢中的作用和在基因调节中的潜在作用.
假设这些转换会影响翻译组,或是被活跃翻译的mRNAs的集合.

研究的目的:

为了研究光暗转换过程中翻译组的快速重编程.
确定GAPC1和GAPC2在调节这些过渡的转基因组中的作用.
为了比较野生类型阿拉比多普西斯的转基因变化与gapc1/c2双击下突变.

主要方法:

多聚体关联转录的RNA测序.
代谢物概况.代谢物概况.
野生类型和gapc1/c2突变植物Arabidopsis之间的比较.

主要成果:

在光过渡点观察到多元体相关转录的重大变化,特别是在gapc1/c2突变.
代谢物概况显示,白天和夜晚转变时,野生类型和gapc1/c2突变物之间的代谢物水平存在差异.
在gapc1/c2突变体中,最显著的翻译组重组发生在夜晚的开始.

结论:

在Arabidopsis中,GAPC1/2在光/黑暗过渡期间控制翻译组起着至关重要的作用.
这项研究支持了GAPC1/2参与这些关键日常事件期间的转录后调节的假设.
破坏GAPC1/2功能会导致显著的代谢和转化重编程.

关键词:

在 GAPC1/C2 中.有关RNA结合的RNA.甘甲基-3-酸盐脱酶.进行代谢物概况分析.月亮照亮功能功能.夜晚黑暗的过渡多元体 (卸载) 卸载翻译ome可以翻译ome.

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