颜色标准化和污点强度校准全幻灯片基于图像的免疫组织化学评估
Chie Ohnishi1,2, Takashi Ohnishi1, Kareem Ibrahim1
1Department of Pathology and Laboratory Medicine, Memorial Sloan Kettering Cancer Center, 1133 York Avenue, New York, NY 10065, USA.
概括
本研究引入了一种校准幻灯片方法,以标准化人类表皮生长因子受体2 (HER2) 免疫组织化学 (IHC) 染色. 这种方法显著降低了用于乳腺癌诊断的全幻灯片成像 (WSI) 分析的可变性.
科学领域:
- 数字病理学数字病理学
- 生物医学成像技术 生物医学成像技术
- 癌症的诊断 癌症的诊断
背景情况:
- 通过全幻灯片成像 (WSI) 来自动量化HER2免疫组织化学 (IHC) 旨在减少主观评估.
- 染色强度和扫描设备的变化在WSI分析中引入了不一致性.
- 准确的HER2评估对于乳腺癌治疗决策至关重要.
研究的目的:
- 为 HER2 IHC 分析开发和验证减轻 WSI 中颜色变异的方法.
- 提高数字病理学中自动HER2评分的可靠性和可重复性.
主要方法:
- 开发了一种带有酸涂层微珠的校准幻灯片,用于与组织样本一起染色.
- 来自微珠的3,3'-diaminobenzobenzidine (DAB) 颜色强度被用来校准WSI颜色变化.
- 应用了两种校准方法:调整分类值和纠正WSI颜色强度.
- 一个现成的图像分析工具被用于基于膜染色的自动细胞分类.
主要成果:
- 拟议的协议证明有效地减少了HER2 IHC WSI的颜色变异影响.
- 在HER2评估中的不一致性被最小化为120个测试中的只有一个幻灯片.
- 该方法在不同的染色日和样品类型中被证明是可靠的.
结论:
- 校准器幻灯片方法提供了一种可靠的方法,可以使用WSI标准化HER2 IHC量化.
- 这种技术提高了自动HER2评估的准确性和一致性,支持临床决策.
- 对WSI染色的标准化对于在病理学中广泛采用自动图像分析至关重要.
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