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塔莫西芬在MCF-7有机体中诱导铁

Lei Ye¹, Fei Zhong¹, Shishen Sun¹

¹Foshan Clinical Medical School, Guangzhou University of Chinese Medicine, Foshan, China.

Journal of cancer research and therapeutics

|December 29, 2023

概括

塔莫西芬通过铁死诱导乳腺癌细胞死亡. 这项研究使用有机体来揭示铁化.

科学领域:

在瘤学瘤学.
细胞生物学细胞生物学
生物化学生物化学

背景情况:

乳腺癌是全球女性的主要恶性瘤.
铁死是一种受调节的细胞死亡形式,与乳腺癌的发展和治疗有关.
与传统的二维培养相比,三维 (3D) 器官模型为研究乳腺癌提供了一个更类似于体内环境的环境.

研究的目的:

通过使用MCF-7有机物来研究他莫西芬在治疗乳腺癌中的机制.
为了探索铁死在他莫西芬对乳腺癌的疗效中的作用.

主要方法:

在3D中培养的乳腺癌MCF-7器官,用tamoxifen治疗的MCF-7细胞和对tamoxifen耐药的MCF-7 (MCF-7 TAMR) 细胞.
利用了转录组测序,GPX4和SLC7A11蛋白质水平的西方涂抹,并对ATP,谷氨和铁离子含量进行分析.
使用细胞计数套件-8 (CCK-8) 试验评估细胞活力.

主要成果:

塔莫西芬显著抑制了MCF-7有机体的生长,并诱导了铁亡.
一种铁灭抑制剂逆转了他莫西芬对MCF-7有机体的抑制作用.
一种铁灭激活剂增强了他莫西芬对MCF-7 TAMR细胞的抑制作用.

结论:

铁化是乳腺癌有机体中因他莫西芬诱导的细胞死亡的关键机制.
这些发现为精确的乳腺癌治疗策略提供了一种基于器官的新方法.

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