相关实验视频

Updated: Jul 6, 2025

Dried Blood Spots - Preparing and Processing for Use in Immunoassays and in Molecular Techniques

Dried Blood Spots - Preparing and Processing for Use in Immunoassays and in Molecular Techniques

Published on: March 13, 2015

一个有趣的例子是,B型肝炎表面抗原在献血者血液中分离出了假阴性

Nevenka Bujandric^1,2, Jasmina Grujic^1,2, Mirjana Krga Milanovic²

¹Department of Transfusiology, Faculty of Medicine University in Novi Sad, Novi Sad, Serbia.

|December 29, 2023

概括

一位献血者最初因罕见突变而对乙型肝炎表面抗原 (HBsAg) 呈阴性. 这一案例凸显了对敏感查试验的需要,以检测献血中的HBsAg变体.

科学领域:

肝病学肝病学是一种肝病学.
病毒学病毒学
免疫学免疫学免疫学

背景情况:

可靠检测乙型肝炎表面抗原 (HBsAg) 突变形式对于献血查至关重要.
研究了一种商业HBsAg查试验中单个假阴性结果的病例.

研究的目的:

为了调查和描述一个虚假阴性HBsAg查结果导致献血者的病例.
为了确定在HBsAg检测中分析灵敏度降低的原因.

主要方法:

常规HBsAg和乙型肝炎病毒 (HBV) DNA测试使用迷你池核酸测试 (MP-NAT).
个人捐赠ID-NAT,量化,测序,并通过替代血清学检测进行评估.
分析之前和之后的捐款,以确认事件的孤立性质.

主要成果:

捐赠者的捐赠最初是HBsAg负的,但HBV DNA是阳性的.
进一步的测试证实了HBsAg阳性,IgM抗HBc阳性和可检测的HBVDNA.
测序揭示了基因型D菌株与特定突变 (T118A,P120T,P127T) 在目标表位附近.

结论:

鉴定出HBsAg突变的独特组合是假阴性查结果的可能原因.
这种突变组合可能会降低某些免疫试验单克隆的分析灵敏度.
这些发现强调了强大的查试验的重要性,以检测血液安全中的HBsAg变体.

关键词:

对DNA突变分析进行分析.献血者献血者献血者献血者献血者乙型肝炎的表面抗原.

更多相关视频

Author Spotlight: Advancements and Challenges in Hepatitis B Virus Detection

Author Spotlight: Advancements and Challenges in Hepatitis B Virus Detection

Published on: December 15, 2023

Detection of Low Copy Number Integrated Viral DNA Formed by In Vitro Hepatitis B Infection

Detection of Low Copy Number Integrated Viral DNA Formed by In Vitro Hepatitis B Infection

Published on: November 7, 2018

相关概念视频

Blood Transfusion and Agglutination

Blood Transfusion and Agglutination

Blood transfusion is a therapeutic measure to restore the blood volume after extensive blood loss due to an accident or a medical procedure. Blood transfusion involves drawing a certain amount of blood from a suitable donor and infusing it into the recipient.
History
The history of blood transfusion dates back to the 17th century, when early attempts were made in animals. In 1818 James Blundell, a British doctor, performed the first successful human blood transfusion. Later in 1900, Karl...

Blood Typing

Blood Typing

Understanding an individual's blood group is a critical component of transfusion medicine. It ensures compatibility in blood transfusions, organ transplants, and even during pregnancy. Determining these blood groups involves the ABO and Rh blood typing systems, utilizing specific antigens and corresponding anti-sera to identify an individual's blood type.
Antigens are protein molecules that reside on the surface of red blood cells (RBCs). The ABO and Rh blood typing systems target...