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相关概念视频

Caspases01:24

Caspases

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Caspase, a family of cysteine proteases, serve as effectors in apoptosis. The ced3 gene in C.elegans was first identified to be involved in apoptosis. This gene encodes the ced-3 caspase that is similar to the interleukin-1-beta converting enzyme or ICE in mammals. In addition to apoptosis, caspases also function in the inflammatory response. Inflammatory caspases are essential in activating pro-inflammatory cytokines that recruit immune cells and block the replication of pathogens inside...
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Actin Filament Depolymerization01:19

Actin Filament Depolymerization

3.1K
Actin filaments (F-actin) are composed of actin subunits. The dissociation of actin monomers can occur from either end of F-actin. The rate of dissociation is faster from the minus-end or the pointed end, where the actin subunits exist with a bound ADP, together known as ADP-actin. The depolymerization of F-actin is aided by proteins, including the actin-depolymerizing factor (ADF) and cofilin family of proteins, gelsolin, and glia maturation factor (GMF).
In F-actin, the ADF/cofilin proteins...
3.1K
The Contractile Ring02:15

The Contractile Ring

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Contractile rings are composed of microfilaments and are responsible for separating the daughter cells during cytokinesis. Contractile ring assembly proceeds along with other cell cycle events; however, very few mechanistic details are known about the timing and coordination of the contractile rings with the cell cycle.
A small GTPase, RhoA, controls the function and assembly of the contractile ring. RhoA belongs to the Ras superfamily of proteins. The activation of formins by RhoA promotes...
6.4K
Determining the Plane of Cell Division02:13

Determining the Plane of Cell Division

3.3K
Positioning the cell division plane is a critical step during development and cell differentiation, particularly during mitosis when the plane is essential for determining the size of the two daughter cells. The cell division plane is perpendicular to the plane of chromosome segregation, but different types of organisms have different cell division mechanisms to suit their morphology and function. 
Animal cells
In animal cells, the cleavage furrow forms along the plane of cell division...
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Cell Motility through Blebbing01:16

Cell Motility through Blebbing

1.9K
Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...
1.9K
Cytoskeletal Coordination in Cell Migration01:32

Cytoskeletal Coordination in Cell Migration

4.8K
A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker...
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相关实验视频

Updated: Jul 6, 2025

Spatiotemporal Analysis of Cytokinetic Events in Fission Yeast
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Spatiotemporal Analysis of Cytokinetic Events in Fission Yeast

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卡斯巴-4在表皮细胞的细胞分裂中通过actin脱聚合起作用.

Kazuhiro Sumida1, Tomomitsu Doi2, Kunie Obayashi2

  • 1Third Department of Internal Medicine, University of Occupational and Environmental Health, Kitakyushu, Japan; Department of Molecular Biology, University of Occupational and Environmental Health, Kitakyushu, Japan.

Biochemical and biophysical research communications
|December 29, 2023
PubMed
概括

卡斯帕-4 (CASP4) 对于细胞分裂至关重要. 它的缺失会导致线粒分裂中的错误,通过破坏actin动力学来影响细胞增殖和DNA含量.

关键词:
在Actin中的Actin是Actin.卡斯帕塞斯 (Caspase) 是一个细胞分裂 细胞分裂科菲林是指一种可菲林.线粒分裂 (mitosis) 是一种发生在细胞间的过程.

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相关实验视频

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Spatiotemporal Analysis of Cytokinetic Events in Fission Yeast
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科学领域:

  • 细胞生物学 细胞生物学
  • 分子生物学分子生物学
  • 生物化学 生物化学

背景情况:

  • 卡斯帕-4 (CASP4) 已知在热,细胞因子成熟,虫体-溶解体融合和细胞迁移中发挥作用.
  • CASP4在细胞分裂中的功能以前没有被阐明.

研究的目的:

  • 研究CASP4在上皮细胞细胞分裂过程中的作用.

主要方法:

  • 使用CRISPR-Cas9基因编辑来创建CASP4淘汰赛 (CASP4 KO) HepG2细胞.
  • 分析了细胞增殖,细胞大小和多核.
  • 检查了线粒细胞的进展,形形成,染色体分离和DNA含量.
  • 通过法洛伊丁染色,评估了丝状活性蛋白水平和活性蛋白脱聚合.
  • 量化了关键的与行为蛋白聚合相关的蛋白质 (ROCK1,LIMK1,p-cofilin) 的表达.

主要成果:

  • CASP4 KO细胞表现出延迟的增殖,细胞大小的增加和多核化.
  • CASP4 KO细胞中的线粒异位缺陷包括多极,异常的定位和染色体分离错误,导致DNA含量增加.
  • 罗伊丁染色显示了CASP4KO细胞中纤维状活性蛋白的增加,这是由于活性蛋白脱聚合功能受损.
  • 在CASP4KO细胞中观察到高水平的动氨酸聚合调节剂 (ROCK1,LIMK1,酸化科菲林).

结论:

  • CASP4在确保适当的细胞分裂方面发挥着至关重要的,以前未知的作用.
  • CASP4通过影响actin脱聚合和维护细胞骨完整性来调节细胞分裂.
  • 干扰CASP4功能会导致显著的线粒异常,并影响细胞增殖.