多能干细胞标记物的简短历史
Peter W Andrews1, Paul J Gokhale1
1The School of Biosciences, The University of Sheffield, Western Bank, Sheffield S10 2TN, UK.
Stem cell reports
|December 29, 2023
概括
识别多能干细胞 (PSC) 依赖于分子标记,但目前的选择,如特定阶段的胚胎抗原 (SSEA) 和转录因子 (OCT4,NANOG,SOX2),并不是PSC独有的. 它们的表情表明茎性,不一定是多能性.
科学领域:
- 干细胞生物学 干细胞生物学
- 分子生物学分子生物学
- 细胞分化的细胞分化.
背景情况:
- 多能干细胞 (PSC) 对于发育和再生研究至关重要.
- 识别和监测PSC通常包括分析特定分子标记物的表达.
- 通常使用的标记物包括细胞表面抗原和转录因子.
研究的目的:
- 审查用于识别多能干细胞 (PSC) 的常用分子标记物.
- 讨论这些标记物的特征和局限性,例如特定阶段胚胎抗原 (SSEA) -1,SSEA -3,SSEA -4,TRA-1 -60,TRA-1 -81,GCTM2,POUF5/OCT4,NANOG和SOX2.
- 突出在假定PSCs的背景下解释标记表达数据的关键考虑因素.
主要方法:
- 文献综述和有关PSC标记物的现有数据的摘要.
- 分析关键细胞表面抗原和转录因子的表达模式和特征.
- 讨论标记物表达对多能性评估的影响.
主要成果:
- 没有一个单一的标记分子是PSCs独特表达的.
- 一些广泛使用的标记物,包括SSEA-1,SSEA-3,SSEA-4,TRA-1-60,TRA-1-81,GCTM2,OCT4,NANOG和SOX2,也被非多能干细胞表达.
- 这些标记物的表达表明了干性,但不一定是多能性.
结论:
- 目前的分子标记物不足以确定多能性.
- 在评估假定的PSC时,对标记表达的仔细解释至关重要.
- 可能需要进一步的研究来确定多能性更具体的标志物.
更多相关视频
08:25Reprogramming Human Somatic Cells into Induced Pluripotent Stem Cells iPSCs Using Retroviral Vector with GFP
Published on: April 3, 2012
20.5K
09:07Chemical Reversion of Conventional Human Pluripotent Stem Cells to a Naïve-like State with Improved Multilineage Differentiation Potency
Published on: June 10, 2018
10.0K
相关概念视频
Induced Pluripotent Stem Cells
4.1K
Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
Somatic...
4.1K
Chromatin Modification in iPS Cells
1.7K
Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
1.7K
Maintenance of the ES Cell State
2.2K
The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
2.2K
Somatic to iPS Cell Reprogramming
2.2K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.2K
Lineage Commitment
3.0K
Commitment is the process whereby stem cells:
3.0K
Methods of Nuclear Reprogramming
1.8K
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
1.8K
