在Bacillus subtilis中,翻译后分泌压力调节是由细胞内和细胞外蛋白酶控制的
Ayşegül Öktem1, Dicky A Pranoto1, Jan Maarten van Dijl1
1Department of Medical Microbiology, University of Groningen, University Medical Center Groningen, the Netherlands.
New biotechnology
|December 29, 2023
概括
消除Bacillus subtilis中的特定蛋白酶可以通过减少产品降解来增强工业酶生产. 这项研究揭示了细胞内蛋白酶是改善微生物工厂中重组蛋白质产量的关键目标.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 分子生物学分子生物学
背景情况:
- 细菌细菌是工业酶生产的关键宿主.
- 蛋白质分泌受到蛋白酶和分泌应激的限制.
- 基因工程旨在改善B. subtilis.中的蛋白质生产.
研究的目的:
- 研究细胞和细胞外蛋白酶在B. subtilis蛋白质分泌中的作用.
- 确定蛋白酶消除对分泌应激反应的影响.
- 确定增强重组蛋白质生产的新目标.
主要方法:
- 野生型和经过基因组缩小的B. subtilis菌株的比较分析.
- 蛋白酶活动测定.
- 对分泌应力效应蛋白水平 (HtrA,HtrB) 的分析.
- 葡萄球菌抗原 (IsaA) 降解的量化.
主要成果:
- 基因组缩小的B. subtilis菌株显示,葡萄球菌抗原的产生有所改善.
- 消除与HtrA和HtrB水平升高相关的AprX (细胞质) 和AprE (细胞外) 蛋白酶.
- 无论是AprX还是AprE都在细胞外降解了葡萄球菌抗原IsaA.
- 细胞内蛋白酶AprX表现出细胞外活性,影响产品水平.
结论:
- 细胞和细胞外蛋白酶的协同作用调节分泌应激因子的分泌.
- 细胞内蛋白酶是改善B. subtilis.重组蛋白质生产的重要目标.
- 消除特定的蛋白酶提供了一种提高微生物细胞工厂性能的策略.
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