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开发和应用LC-MS/MS方法来量化眼睛中硫化的含量

Anthonia Okolie¹, Maria Rincon Nigro², Sharhazad Polk¹

¹Department of Pharmaceutical Sciences, College of Pharmacy and Health Sciences, Texas Southern University, Houston, TX, 77004, USA.

Analytical biochemistry

|December 29, 2023

概括

这项研究开发了一种敏感的LC-MS/MS方法,用于测量眼组织中的硫化 (H2S). 这种新方法成功量化了用脂多糖化物治疗的猪虹膜-毛体中的H2S变化.

科学领域:

眼科医生眼科眼科
分析化学分析化学
生物化学生物化学

背景情况:

眼睛组织中硫化 (H2S) 的生理水平没有得到充分的记录.
目前用于H2S测量的UV/Vis光谱法缺乏灵敏度和精度.

研究的目的:

开发和验证一种灵敏而强大的前列导化液体染色学-并联质谱法 (LC-MS/MS) 方法.
测量H2S水平在孤立的猪眼组织中的变化.

主要方法:

通过使用单二胺来衍生H2S,形成硫化二胺 (Sdb).
用LC-MS/MS进行量化,在水的纯度BEH C18列上监测多离子反应 (MRM).
该方法在灵敏度和精度方面得到了验证.

主要成果:

开发的LC-MS/MS方法在H2S量化方面表现出高灵敏度和稳定性.
该方法已成功应用于测量H2S水平在孤立的猪虹膜-皮体 (ICB) 组织中.
在ICB组织中评估了H2S水平,这些组织暴露在不同度的脂多糖 (LPS) 中.

结论:

经过验证的LC-MS/MS方法为量化眼组织中的H2S提供了合适的方法.
这种技术可以在炎症条件下研究眼组织中的H2S动态,例如LPS治疗.

关键词:

衍生化衍生化衍生化硫化是一种硫化.炎症炎症是一种炎症.这是LC-MS/MS.一氧化二氧化 (Monobromobimane) 是一种眼睛研究眼睛研究

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