从不同的牛奶来源分离,鉴定Weissella confusa和Lactococcus lactis的特征,并确定益生菌特征
Melda Onur1, Harun Önlü2,3
1Ministry of Agriculture and Forestry, Istanbul, Türkiye.
概括
来自牛奶来源的乳酸细菌 (LAB) 显示出有前途的益生菌潜力. 这些强壮的菌株在压力下表现出极好的生存能力,强大的粘附性,并且安全使用.
科学领域:
- 微生物学 微生物学
- 食品科学 食品科学 食品科学
- 益生菌 益生菌 益生菌
背景情况:
- 乳酸细菌 (LAB) 在发酵食品中至关重要,并因其健康益处而闻名.
- 从不同的牛奶来源研究新的LAB菌株是发现新益生菌的关键.
研究的目的:
- 评估来自各种牛奶来源的LAB分离物的益生菌性质.
- 为了识别和描述LAB菌株的功能性食品成分潜力.
主要方法:
- 通过使用16S rRNA基因测序来确定LAB分离物.
- 益生菌的潜力通过生存测试 (低pH,素,胆盐),粘附测试 (细胞粘附,疏水性,聚合) 和安全评估 (血液溶解,凝酶,DNase活动) 来评估.
主要成果:
- 鉴定出了四种乳球菌乳酸菌和两种韦塞拉混菌株.
- 在LAB分离物体中,表现出对胆盐和低pH值的显著耐药性,在素中生存率高.
- 所有菌株都表现出强烈的自我聚合,对病原体的适度联合聚合,以及对Caco-2细胞的良好粘附. 没有检测到血液溶解剂,凝酶或DNase活性.
结论:
- 研究的LAB菌株具有显著的益生菌特性,包括耐压力和抑制病原体.
- 这些发现支持这些LAB作为食品中的益生菌的潜在应用.
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