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在溶液中的碳纳米管上绘制DNA形态图,使用X射线散射干扰计

Daniel J Rosenberg^1,2,3, Francis J Cunningham⁴, Joshua D Hubbard⁴

¹Graduate Group in Biophysics, University of California, Berkeley, Berkeley, California 94720, United States.

Journal of the American Chemical Society

|December 30, 2023

概括

在单壁碳纳米管 (SWCNT) 上测量溶液中单链DNA (ssDNA) 的X射线散射干扰度. 这揭示了ssDNA结构如何随着多巴胺结合而变化,这对于纳米传感器设计至关重要.

科学领域:

纳米技术
生物物理
材料科学

背景情况:

用单链DNA (ssDNA) 功能化的单壁碳纳米管 (SWCNT) 是生物应用的有希望的纳米传感器.
了解溶液中SWCNT的ssDNA构造对于传感器的功能至关重要,但仍然具有挑战性.
现有的方法通常依赖于计算模型或脱水条件,而不是反映生物环境.

研究的目的:

开发和应用X射线散射干扰仪 (XSI) 来直接测量SWCNT溶液中的ssDNA几何.
量化ssDNA形态和形状变化以应对环境因素和分析物结合.
提供基于SWCNT的纳米传感器传感机制的见解.

主要方法:

在ssDNA功能化的SWCNT上使用金纳米粒子 (AuNP) 标签的X射线散射干扰测量 (XSI).
在不同的离子强度下测量了不同寡合体长度 (GT) 6和 (GT) 15的ssDNA构造.
在与多巴胺相互作用时研究了ssDNA的结构变化.

主要成果:

量化了不同的ssDNA形态: (GT) 6采用了更有序的环结构,而 (GT) 15显示了更广泛的安排.
在多巴胺结合时观察到ssDNA形状变化,包括轴延长和辐射收缩.
证明了XSI在溶液相聚合物纳米管系统中探测ssDNA结构的能力.

结论:

在生物相关的水性环境中,XSI提供了一种直接的方法来测量SWCNT上的ssDNA构造.
在分析物相互作用时ssDNA结构及其动态变化是SWCNT纳米传感器性能的关键.
这种方法可以指导基于纳米粒子的先进生物传感器的合理设计.

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