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神经元氧化合成酶表达在卫星细胞衍生的初级肌细胞分化过程中增加

Masaki Kusano¹, Kazuya Sakai², Yuki Tomiga²

¹The Fukuoka University Institute for Physical Activity, Fukuoka University, 8-19-1 Nanakuma, Jonan-ku, Fukuoka 814-0180, Japan. masaki.1202.jump@gmail.com.

Cellular and molecular biology (Noisy-le-Grand, France)

|December 30, 2023

概括

神经元氧化合成酶 (nNOS) 在肌肉干细胞中表达,其亚细胞局部在分化过程中发生变化,这表明它在肌肉发育中的作用. 诺斯1的DNA甲基化保持稳定.

科学领域:

肌肉干细胞生物学肌肉干细胞生物学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
骨肌肉生理学骨肌肉生理学

背景情况:

神经元氧化合成酶 (nNOS) 对于成人肌肉骨肌肉质量调节至关重要.
在分化过程中,nNOS在肌肉干细胞中的功能尚不清楚.
肌肉卫星细胞对于肌肉生长,维护和再生至关重要.

研究的目的:

在分化过程中研究肌肉卫星细胞衍生的肌细胞体中的nNOS表达和DNA甲基化.
为了澄清nNOS在肌肉干细胞分化中的作用.

主要方法:

免疫组织化学检测nNOS和Pax7.
对No1μ mRNA水平的分析.
在未分化和分化肌细胞中评估NOS1DNA甲基化水平.

主要成果:

nNOS在未分化和分化肌肉卫星细胞中表达.
在未分化的细胞中,nNOS与Pax7共局,并在分化的细胞中转移到细胞质中.
在分化过程中,Nos1μ mRNA水平增加,而NOS1 DNA甲基化保持不变.

结论:

nNOS表达和改变的亚细胞定位与肌肉干细胞分化有关.
通过NOS1的DNA甲基化进行表观遗传调节似乎无法控制在分化过程中发生的nNOS变化.
nNOS可能在肌肉卫星细胞的分化过程中发挥功能作用.

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