NAC61调节葡萄藤中晚期和成熟后的透,氧化和生物应激反应
Chiara Foresti1, Luis Orduña2, José Tomás Matus2
1Department of Biotechnology, University of Verona, Verona, Italy.
Journal of experimental botany
|December 30, 2023
概括
葡萄藤NAC61转录因子调节果的成熟,脱水和应激反应. 它控制糖度,并与NAC60相互作用,激活关键的成熟基因.
科学领域:
- 植物分子生物学 植物分子生物学
- 植物生理学 植物生理学
- 农业科学 农业科学
背景情况:
- 葡萄成熟过程涉及复杂的生化和生理变化.
- 控制葡萄成熟的分子机制,特别是在晚期和成熟后的阶段,尚未完全理解.
- 转录因子在调节植物发育和应激反应方面发挥着至关重要的作用.
研究的目的:
- 研究葡萄藤NAC转录因子NAC61在调节葡萄成熟中的作用.
- 在葡萄发育和收获后的过程中识别NAC61调节的基因和途径.
- 阐明NAC61与葡萄藤中的其他调节因素的相互作用.
主要方法:
- 在葡萄成熟和收获后脱水期间分析NAC61表达模式.
- 在 *Nicotiana benthamiana* 中表达NAC61的子宫外表达,以研究生理效应.
- 对葡萄树叶过度表达NAC61.1的转录组分析 (RNA-Seq)
- 用DNA亲和力净化和测序 (DAP-seq) 来识别直接的NAC61目标.
- 对基因调节和蛋白质与蛋白质相互作用的验证 (例如,与NAC60).
主要成果:
- 在收获后脱水期间,NAC61的表达率很高,与葡萄中的糖度相关.
- 在 *N. benthamiana* 中的异卵性 NAC61 表达诱导了生理变化,包括减少口腔导电性和组织崩.
- NAC61直接调节涉及stilbenoid代谢 (MYB14),透应激 (DHN1b) 和生物应激 (*WRKY52*) 的基因.
- NAC61与NAC60相互作用,共同激活MYB14和NAC61.1的表达.
结论:
- NAC61是一种关键的转录因子,调节葡萄的成熟,脱水和相关的压力反应.
- NAC61在一个调节网络中起作用,控制着斯蒂尔贝诺因代谢,透性,氧化性和生物应激耐受性.
- NAC61和NAC60之间的相互作用凸显了葡萄藤成熟的复杂监管机制.
关键词:
这种植物是Botrytis cinerea.在 NAC61 中,它被认为是NAC61.无生物应激应激是一种非生物应激.生物压力是生物压力.葡萄树的葡萄藤是葡萄树的葡萄.晚期成熟 晚期成熟收获后脱水脱水 收获后脱水斯蒂尔贝诺伊德的代谢过程更多相关视频
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