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circELP2逆拼接生物发生,并通过准线粒体质量控制途径作为一种亲纤维化因子发挥作用

Songzi Zhang^1,2, Diwei Tu¹, Weili Liu¹

¹Department of Respiratory and Critical Care Medicine, Binzhou Medical University Hospital, Binzhou Medical University, Binzhou, China.

Journal of cellular and molecular medicine

|December 30, 2023

概括

阻断circELP2,一个圆形RNA,可以通过向miR-630-YAP1/TAZ通路来缓解肺纤维化. 这一发现表明circELP2是治疗肺纤维化的潜在治疗标.

科学领域:

分子生物学分子生物学
细胞生物学细胞生物学
肺部医学肺部医学

背景情况:

异形性肺纤维化 (IPF) 是一种慢性肺病,其机制尚不清楚.
纤维细胞激活和细胞外基质沉积是肺纤维化的主要特征.

研究的目的:

为了研究circELP2在肺纤维化中的作用.
阐明circELP的生物发生和调节机制2.
评估circELP2作为IPF的治疗点.

主要方法:

研究了由异质核核核糖核蛋白L.启动的circELP2生物发生.
研究了circELP2在纤维细胞增殖,迁移和细胞外基质沉积中的功能.
使用机理学研究来探索circELP2-miR-630-YAP1/TAZ通路.
在小鼠体内的体内治疗评估中使用了腺病毒载体.

主要成果:

circELP2通过激活纤维细胞增殖和细胞外基质沉积来促进肺纤维化.
细胞质圈ELP2海绵miR-630,上调YAP1和TAZ的调节.
YAP1/TAZ调节线粒体质量控制通路的基因 (mTOR,Raptor,mLST8).
在体内阻断circELP2通路可缓解小鼠肺纤维化.

结论:

circELP2在促进肺纤维化方面发挥着至关重要的作用.
线粒体质量控制途径circELP2-miR-630-YAP1/TAZ-线粒体质量控制途径是肺纤维化的一个关键机制.
阻断circELP2为治疗肺纤维化提供了一个潜在的治疗策略.

关键词:

雅普1 / 塔兹环RNA 环RNA 是一个环RNA.这是一个小RNARNA.线粒体质量控制途径肺部纤维化肺部纤维化

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