马尔特1蛋白酶通过口腔状平原的mTOR途径调节T细胞免疫力
Xiao-Feng Wang1, Fang Wang1,2, Gang Zhou3,4
1State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Wuhan, China.
Inflammation
|December 30, 2023
概括
粘膜相关的淋巴组织淋巴瘤转位蛋白1 (MALT1) 的表达在口腔平平流体 (OLP) T 细胞中发生变化. 抑制MALT1和mTOR通路可以抑制T细胞的增殖,并促进OLP的亡.
科学领域:
- 免疫学 免疫学 免疫学
- 细胞生物学 细胞生物学
- 口腔医学是指口腔医学.
背景情况:
- 口腔平 (OLP) 是一种T细胞介导的粘膜疾病,其病因不明.
- 粘膜相关的淋巴组织淋巴瘤转位蛋白1 (MALT1) 在OLP T细胞功能障碍中的作用尚不清楚.
研究的目的:
- 为了研究OLP T细胞中的MALT1表达.
- 探索MALT1和拉巴胺素 (mTOR) 的机械标对OLP的T细胞免疫力的影响.
主要方法:
- 免疫组织化学,多重免疫组织化学和流细胞测量被用来分析MALT1的表达.
- 西部斑点,CCK8测定和流动细胞计量评估了MALT1和mTOR对T细胞免疫力的影响.
- 用OLP血或细胞因子刺激Jurkat T细胞以评估MALT1表达变化.
主要成果:
- 在局部OLP T细胞中,MALT1表达升高,但在外围OLP T细胞,MAIT细胞和MAITfh细胞中降低.
- 抑制MALT1 (MI-2) 增加了mTOR酸化,BCL10表达,并促进了T细胞亡,同时抑制了增殖.
- 结合MI-2和拉巴胺治疗进一步抑制了T细胞增殖和增强了细胞亡.
结论:
- 在OLP患者的局部病变和外周血液中,MALT1的表达失调.
- 抑制mTOR通路可以增强MALT1抑制对T细胞增殖和亡的影响.
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