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精子蛋白质格局在繁殖公牛中发生了变化,精子DNA碎片化指数更高

Kathan Raval¹, Arumugam Kumaresan¹, Manish Kumar Sinha¹

¹Theriogenology Laboratory, Southern Regional Station of ICAR-National Dairy Research Institute, Bengaluru, 560030, Karnataka, India.

|December 30, 2023

概括

在公牛中,高精子DNA碎片化 (DFI) 与改变的精子蛋白有关,这些蛋白质对生育至关重要. 这项研究确定了在高DFI公牛中影响容量,精子生成和DNA修复的特定蛋白质变化.

科学领域:

生殖生物学生殖生物学
蛋白质组学是指蛋白质组学.
动物科学动物科学

背景情况:

精子DNA损伤是不孕症的一个已知的因素.
精确的分子机制将精子DNA损伤与生育结果联系起来需要进一步调查.
精子蛋白对于受精和随后的胚胎发育至关重要.

研究的目的:

为了确定高精子DNA碎片化指数 (DFI) 的公牛精子中的蛋白质变化.
了解精子DNA损伤如何影响繁殖公牛的生育能力的分子基础.

主要方法:

来自50只荷尔斯坦-弗里西亚杂交公牛的精液样本使用精子染色质结构试验进行了分析.
牛被分为高DFI (HDFI) 和低DFI (LDFI) 组 (每组n=6).
对两组精子进行了高通量蛋白质组分析 (液体染色学和质谱学).

主要成果:

在公牛精子中,共发现了4567种蛋白质.
在HDFI和LDFI组之间观察到蛋白质表达的显著差异,在HDFI组中265种蛋白质上调和262种蛋白质下调.
改变的蛋白质与关键过程有关,包括精子电容,精子生成和DNA修复机制.

结论:

在公牛中,高精子DNA碎片化与精子蛋白质组的显著改变有关.
这些蛋白质变化会影响诸如电容,精子生成和DNA修复等关键功能,可能会影响生育能力.
这些发现为精子DNA损伤和男性生育能力之间的关系提供了分子洞察力.

关键词:

这是一头繁殖公牛.在DNA碎片化过程中,DNA碎片化.生育能力生育能力生育能力蛋白质组学是指蛋白质组学.精子动物 (Spermatozoa) 是一种精子.

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