MAP9/MAPH-9支持轴膜微管双重体,并调节运动运动
Michael V Tran1, Daria Khuntsariya2, Richard D Fetter3
1Department of Biology, Stanford University, Stanford, CA 94305, USA.
Developmental cell
|December 30, 2023
概括
研究人员确定了微管相关蛋白9 (MAP9) 对于维持小体双重体 (MTD) 在小体中的结构至关重要. 失去MAP9会破坏MTD,影响毛功能和运动速度.
科学领域:
- 细胞生物学 细胞生物学
- 结构生物学 结构生物学
- 生物化学 生物化学
背景情况:
- 微管双体 (MTDs) 是多种生物体内的重要组成部分.
- 控制MTD形成和体内维持的精确机制尚未完全阐明.
- 了解MTD结构是理解纤毛功能的关键.
研究的目的:
- 为了识别与微管双重体 (MTD) 相关的蛋白质.
- 研究微管相关蛋白9 (MAP9) 在MTD结构和功能中的作用.
- 探索MAP9在调节状电机和MTD完整性的保存功能.
主要方法:
- 使用C. elegans MAPH-9进行蛋白质识别和局部化研究.
- 电子显微镜用于评估MTD的超结构.
- 功能性测试用于评估毛运动速度和毛功能.
- 在细胞培养和小鼠组织中分析哺乳动物MAP9局部化.
主要成果:
- 微管相关蛋白9 (MAP9) 被确定为一种MTD相关蛋白.
- C. elegans MAPH-9局部化到MTDs,而氨酸聚氨基化有助于这种特异性.
- 丢失MAPH-9导致MTD缺陷,包括改变的B管结构和减少的原纤维数量.
- MAPH-9 缺乏导致轴膜运动速度失调和毛功能受损.
- 哺乳动物MAP9局部化到轴膜,这表明它保留了作用.
结论:
- MAP9/MAPH-9对于保持轴膜微管双体 (MTD) 的结构完整性至关重要.
- 这种蛋白质在调节纤毛运动功能的过程中起着保留作用.
- 在不同物种中,MAP9/MAPH-9对于适当的纤毛运动和整体纤毛功能至关重要.
相关概念视频
Microtubules in Cell Motility
3.3K
Microtubules are thick hollow cylindrical proteins that help form the cytoskeleton. Microtubules have varied roles in the cell. These filaments help form cellular appendages like cilia and flagella, which are responsible for locomotion. The cilia arise from basal bodies, separated from the main body by a membrane-like structure forming the transition zone. This zone is the gate for the entry of lipids and proteins, creating a unique composition of lipids and proteins in the ciliary membrane and...
3.3K
Microtubule Associated Motor Proteins
8.0K
Eukaryotic cells have different motor proteins for transporting various cargo within the cell. These motor proteins differ based on the filament they associate with, the direction they move within the cell, and the type of cargo they transport. Motor proteins that associate with microtubules are known as microtubule-associated motor proteins. There are two families of microtubule-associated motor proteins —Kinesins and Dyneins. Both these proteins assist in the transport of cellular...
8.0K
Assembly of Complex Microtubule Structures
1.8K
Complex microtubule structures are present in resting cells and in dividing cells. In resting cells, they are responsible for maintaining the cellular architecture, tracks for intracellular transport, positioning of organelles, assembly of cilia and flagella. They mediate the bipolar spindle assembly for chromosomal segregation and positioning of the cell division plate in dividing cells. The formation of microtubule complex structures depends on the cell type, cell stage, and cell function.
1.8K
Microtubule Associated Proteins (MAPs)
4.3K
Microtubule function and architecture are regulated by an array of specialized proteins called microtubule-associated proteins or MAPs. These proteins are widespread across different organisms and have conserved protein motifs, like the multi-TOG domain for tubulin binding found in the CLASP family of MAPs. Some MAPs are lineage-specific based on their conserved domains. Their functions depend upon the cytoskeletal architecture and cell type they are located within. In-plant cells, a specific...
4.3K
The Movement of Organelles and Vesicles
4.5K
In eukaryotic cells, cytoskeletal filaments such as actin, microtubules, and intermediate filaments form a mesh-like cytoskeletal network. These filaments serve as tracks for transporting cellular cargo. Specialized motor proteins use the chemical energy stored in adenosine triphosphate (ATP) for this transport. During interphase, microtubules are polarized, with the plus-end towards the cell periphery and the minus-end towards the cell center. Two microtubule-associated motor proteins,...
4.5K
Anaphase A and B
4.1K
Microtubules form through the end-to-end polymerization of tubulin heterodimers. Kinetochore microtubules originate from the spindle poles, and their plus-ends connect with the kinetochores on sister-chromatids. Ndc80 protein complexes, present on the kinetochore, form low-affinity links with the plus end of these kinetochore microtubules.
Plus-end depolymerization releases tubulin heterodimers from the terminal region of the microtubule. As tubulin subunits are lost, the Ndc80 complexes detach...
Plus-end depolymerization releases tubulin heterodimers from the terminal region of the microtubule. As tubulin subunits are lost, the Ndc80 complexes detach...
4.1K


