MdbHLH93通过激活MdTyDC表达和促进多巴胺生物合成来赋予干旱耐受性
Xiaomin Liu1, Yanpeng Wang2, Xiaoying Ma1
1State Key Laboratory for Crop Stress Resistance and High Efficiency Production/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A&F University, Yangling 712100, Shaanxi, China.
International journal of biological macromolecules
|December 30, 2023
概括
果植物通过多巴胺合成增强干旱耐受性. 转录因子MdbHLH93调节提氨酸脱碳酶 (TyDC),提高多巴胺水平,改善植物对水资源短缺的抵抗力.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 多巴胺和氨酸脱碳酶 (TyDC) 对植物生长和干旱应激反应至关重要.
- 控制多巴胺在植物干旱耐受性中的作用的分子机制尚未完全理解.
研究的目的:
- 为了阐明马卢斯家属bHLH93 (MdbHLH93) 在调节果中多巴胺生物合成和干旱耐受性的作用.
- 在干旱条件下调查MdbHLH93和Malus domestica TyDC (MdTyDC) 之间的监管关系.
主要方法:
- 在干旱压力下对MdbHLH93的基因表达分析.
- 促进体结合试验以确定MdbHLH93与MdTyDC促进体的相互作用.
- 对MdbHLH93和MdTyDC进行过度表达和干扰测定,以评估它们对多巴胺合成和干旱耐受性的影响.
主要成果:
- 干旱压力显著增加了果植物中的MdbHLH93表达.
- MdbHLH93直接与MdTyDC促进体结合,积极调节其表达,从而增加多巴胺合成.
- 同时过度表达MdbHLH93和MdTyDC增强了多巴胺合成和干旱耐受性,而MdbHLH93干扰减少了这些影响.
结论:
- MdbHLH93通过转录控制MdTyDC表达,积极调节果中的多巴胺积累.
- 通过MdbHLH93-MdTyDC通路调解的多巴胺含量增加,使果植物具有增强的干旱耐受性.
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