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严格的反应在产生抗生素的菌株Streptomyces coelicolor中被强烈激活

Clara Lejeune¹, David Cornu¹, Laila Sago¹

¹Institute for Integrative Biology of the Cell (I2BC), Université Paris-Saclay, CEA, CNRS, Gif-sur-Yvette, France.

Research in microbiology

|December 30, 2023

概括

由于营养压力,Streptomyces lividans和Streptomyces coelicolor表现出不同的代谢能力. 蛋白质组分析揭示了翻译蛋白的差异表达,表明S. coelicolor中强烈的严格反应激活.

科学领域:

微生物学微生物学
分子生物学分子生物学
生物化学生物化学

背景情况:

利维丹斯菌株 (Streptomyces lividans) 和灰色菌株 (Streptomyces coelicolor) 是密切相关的菌株,具有不同的专门代谢物生产.
与S. lividans相比,S. coelicolor是一种强大的抗生素生产者,具有较低的和酸盐同化和较慢的生长率.
营养压力,特别是酸盐的可用性,显著影响微生物生理学和二次新陈代谢.

研究的目的:

在不同的酸盐条件下对S. lividans和S. coelicolor转化器的蛋白质组数据集进行比较分析.
阐明营养压力对蛋白质丰富性的影响以及这些Streptomyces菌株的严格反应.
了解营养同化,生长率和专门的代谢物生产之间的关系.

主要方法:

在低酸盐和高酸盐条件下种植的S. lividans和S. coelicolor的比较蛋白质组分析.
蛋白质丰度的量化,专注于翻译装置.
与严格反应 (例如RelA) 相关的基因表达的分析.

主要成果:

大多数翻译器官蛋白在S. coelicolor中比S. lividans中少得多.
特定的蛋白质,延长因子Tu 3 (ET-Tu 3) 和类似TrmA的甲基转移酶,在S. coelicolor中更为丰富.
与酸盐限制相比,S. lividans中的酸盐充足导致了较高的核糖体蛋白丰度,这表明酸盐限制诱导了严格的反应.

结论:

在S. coelicolor中显示出严格反应的强烈激活,由差异性蛋白质表达证明.
酸盐限制有助于通过RelA表达诱导S. lividans的严格反应.
营养状况显著影响转化机制和严格的反应,影响Streptomyces物种的代谢能力.

关键词:

抗生素的生物合成.规则规则规则规则规则规则这种病名为Streptomyces.严格的反应反应.翻译设备翻译设备 ppGpppp ppGpppp 这是一个很大的问题.

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