从一个开放的读取框架中翻译的两个病毒蛋白针对不同的植物防御层
Yuzhen Mei1, Tao Hu2, Yaqin Wang2
1State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou, Zhejiang 310058, China; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Plant communications
|December 31, 2023
概括
病毒使用翻译泄漏来抑制植物的防御. 番茄金色马赛克病毒C4蛋白质向叶绿体和血膜,降解防御组件并使感染成为可能.
科学领域:
- 植物病原体相互作用
- 分子病毒学分子病毒学.
- 植物免疫力 植物免疫力
背景情况:
- 植物对病原体攻击有多层次的防御反应.
- 病毒采用策略来克服宿主防御,包括最大限度地提高基因组编码能力.
- 抑制宿主防御对于病毒感染和传播至关重要.
研究的目的:
- 调查翻译泄漏作为一种病毒策略来抑制宿主防御.
- 来自番茄金色马赛克病毒的两个病毒效应因子cC4和mC4的机制阐明.
- 了解这些作用因子如何协同抑制植物免疫力.
主要方法:
- 研究了质细胞局部化的C4 (cC4) 和膜相关的C4 (mC4) 病毒因子的功能.
- 分析了cC4和mC4对NbPUB4的招募,以诱导无处不在和降解.
- 研究了单片氧 (O2) 和NbMBS1在激活CERK1基因表达中的作用.
- 研究了mC4对CERK1介导的抗病毒防御的影响.
主要成果:
- cC4诱导叶绿体的降解,并取消叶绿体介导的防御.
- cC4触发单点氧气的产生,导致核CERK1基因的激活.
- mC4降解了CERK1,抑制了基于血的,类似受体的激酶依赖的防御系统.
- 这种翻译泄漏策略在病毒物种中得到保护,并影响宿主范围.
结论:
- 翻译泄漏是一种保存的病毒策略,用于抑制不同的植物抗病毒反应.
- cC4和mC4的合作作用有效地破坏了基于质体和膜的免疫力.
- 工程堆叠的细胞抗病毒反应对持久的作物疾病耐药性充满希望.
相关概念视频
Defenses Against Pathogens and Herbivores
23.7K
Plants present a rich source of nutrients for many organisms, making it a target for herbivores and infectious agents. Plants, though lacking a proper immune system, have developed an array of constitutive and inducible defenses to fend off these attacks.
23.7K
Viral Structure
62.3K
Viruses are extraordinarily diverse in shape and size, but they all have several structural features in common. All viruses have a core that contains a DNA- or RNA-based genome. The core is surrounded by a protective coat of proteins called the capsid. The capsid is composed of subunits called capsomeres. The capsid and genome-containing core are together known as the nucleocapsid.
62.3K
Leaky Scanning
5.1K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.1K
Introduction to Plant Diversity
44.7K
From Water to Land
44.7K
Cell Signaling in Plants
5.6K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.6K
Plant Cell Wall
56.6K
The plant cell wall gives plant cells shape, support, and protection. As a cell matures, its cell wall specializes according to the cell type. For example, the parenchyma cells of leaves possess only a thin, primary cell wall.
56.6K


