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在牛体外受精期间的等离子素激活和等离子素抑制:对受精参数和早期胚胎发育的影响

Gabriela Rizo¹, Antonio Daniel Barrera², Elina Vanesa García²

¹Instituto Superior de Investigaciones Biológicas (INSIBIO), CONICET-UNT, and Instituto de Biología 'Dr. Francisco D. Barbieri', Facultad de Bioquímica, Química y Farmacia, UNT, Chacabuco 461 (4000), San Miguel de Tucumán, Tucumán, Argentina.

Reproductive biology

|December 31, 2023

概括

补充IVF介质的链球激酶 (SK) 并没有显著影响牛胚胎的发育. 然而,用e-aminocaproic acid (ε-ACA) 抑制等离子体活性降低了胚胎发育的成功率.

科学领域:

生殖生物学生殖生物学
胚胎学胚胎学
生物化学生物化学

背景情况:

卵细胞含有等离子体/等离子体系统的组成部分,对于成熟和受精至关重要.
了解这个系统在早期胚胎发育中的作用对于改善体外受精 (IVF) 结果至关重要.

研究的目的:

研究调节等离子素激活和等离子素抑制对牛受精和植入前胚胎发育的影响.
在试管婴儿中评估链球酶 (SK) 和e-aminocaproic acid (ε-ACA) 补充剂的作用.

主要方法:

牛累积卵细胞复合体 (COCs) 在体外成熟.
在试管婴儿介质中进行了授精,并补充了链酶 (SK) 或e-aminocaproic acid (ε-ACA).
分析了受精参数,胚胎分裂率,胚胎细胞产量和基因表达 (SDHA,DNMT3A).

主要成果:

链酶 (SK) 促进了精子区域的透明粘附,但没有改变单精子率.
在SK和对照组中,裂变率和胚胎细胞产量是可比的.
与对照组相比,埃普西隆-氨基甲酸 (ε-ACA) 治疗导致了较低的裂变率和胚胎细胞产量.

结论:

补充IVF介质的链球酶 (SK) 并不会显著损害牛胚胎的质量或发育参数.
用e-aminocaproic acid (ε-ACA) 抑制等离子体活性会对体外胚胎发育产生负面影响.
血原/血活性对于牛的早期胚胎发生成功至关重要.

关键词:

在这个过程中,IVF IVF.塑原激活激活的方法植入前胚胎的胚胎发育情况链球酶是一种链球酶.一个-氨基甲酸.

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