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阿拉比多普西斯·塔利亚纳的类酸盐特异性脂酶C2是Botrytis cinerea的扩散所需的

Luciana Robuschi¹, Oriana Mariani², Enzo A Perk¹

¹Instituto de Investigaciones Biológicas, Consejo Nacional de Investigaciones Científicas y Técnicas, Universidad Nacional de Mar del Plata, 7600 Mar del Plata, Argentina.

Plant science : an international journal of experimental plant biology

|December 31, 2023

概括

脂酶C2 (AtPLC2) 作为阿拉比多普西斯的敏感性基因,促进真菌病原体Botrytis cinerea的扩散. 抑制或突变AtPLC2可以增强植物免疫力,减少病变大小.

科学领域:

植物免疫力植物免疫力
植物病理学植物病理学
脂质信号传递是什么

背景情况:

脂酶C (PLC) 对于植物发育和应激反应至关重要,PLC激活是病原体感知的早期事件.
阿拉比多普西斯塔利亚纳有七个PLC基因,其中AtPLC2是最丰富和构成性表达的异型.
PLC与植物防御有关,特别是在反应性氧物种 (ROS) 生产中,并且已知AtPLC2在口腔免疫期间激活RBOHD用于ROS生成.

研究的目的:

调查AtPLC2在植物抗性中对死菌真菌Botrytis cinerea的作用.
确定AtPLC2是否在植物病原体相互作用中起到敏感性基因的作用.

主要方法:

在Arabidopsis thaliana中使用人工微RNA (amiR PLC2) 进行基因沉默.
对AtPLC2 (plc2-1) 的一个零突变的分析.
对Botrytis cinerea感染的表型分析,包括病变大小测量,ROS产量测定和依赖酸 (SA) 的信号评估.

主要成果:

在PLC2-沉默和plc2-1突变植物表现出减少的Botrytis cinerea病变的发展.
在plc2-1突变体中,在真菌感染后,ROS产量减少.
感染plc2-1植物导致SA依赖信号的强化,这表明病原体操纵了AtPLC2-介导的通路.

结论:

AtPLC2是一种易感性 (S) 基因,它促进了Botrytis cinerea感染和Arabidopsis的扩散.
准AtPLC2触发的反应可能是增强植物对真菌病原体耐药性的策略.

关键词:

阿拉比多普西斯塔利亚纳.这种植物是Botrytis cinerea.脂质信号传递是什么脂酶C是一种脂酶.工厂防御系统的工厂防御系统有反应性氧物种的反应性氧物种.

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