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Updated: Jul 6, 2025

Light-driven Enzymatic Decarboxylation
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对CYP68JX的氧化还原合作伙伴和辅助因子NADPH供应进行工程,以获得高效的类固醇两步排序的选择性氧化活性

Wei Liu1, Hui Li2, Dongxin Guo2

  • 1School of Biotechnology, Jiangnan University, Wuxi 214122, China.

The Journal of steroid biochemistry and molecular biology
|December 31, 2023
PubMed
概括

研究人员使用酵母中的P450氧酶CYP68JX增强了脱氨 (DHEA) 的生物转化. 优化氧化还原系统和辅助因子供应显著提高了目标产品7α,15α-diOH-DHEA的产量.

关键词:
7α,15α-二H-DHEA 的使用.在CYP68IX中使用.辅因子NADPH的存在作为一个Redox合作伙伴,CPR是CPR的合作伙伴.这种植物是Saccharomyces cerevisiae.

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