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相关概念视频

Effects of EDTA on End-Point Detection Methods01:18

Effects of EDTA on End-Point Detection Methods

275
Different methods, such as visual observance of metal-ion indicators, spectroscopic techniques, and potentiometric methods, can determine the endpoint of an EDTA titration.
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a...
275
EDTA: Direct, Back-, and Displacement Titration01:30

EDTA: Direct, Back-, and Displacement Titration

2.8K
The EDTA titration types for metal ion analysis include direct titration, back-titration, and replacement titration.
Direct titration involves buffering the metal ion solution to the desired pH and directly titrating with standard EDTA until the endpoint. The optimum pH ensures a large conditional formation constant of metal−EDTA and visibility of the free indicator color in the solution. In addition, auxiliary complexing reagents are used to prevent the precipitation of metal hydroxides...
2.8K
EDTA: Indirect and Alkalimetric Titration01:23

EDTA: Indirect and Alkalimetric Titration

926
Unlike direct titration, back-titration, and displacement titration, indirect titration is an EDTA titration method for quantifying anions. In the indirect titration method, anions are precipitated as their insoluble salts with excess metal ions. The filtrate containing the excess metal ions is directly titrated with standard EDTA until the endpoint is achieved. Another approach involves extracting the metal ion and back-titrating with standard EDTA to obtain the endpoint. In this way, the...
926

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相关实验视频

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Assaying for Inorganic Polyphosphate in Bacteria
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重新评估基于EDTA的多酸盐量化方法的准确性和特异性.

Xiangnan Zheng1, Shida Li1, Shaokui Zheng1

  • 1School of Environment, MOE Key Laboratory of Water and Sediment Sciences/State Key Lab of Water Environment Simulation, Beijing Normal University, Beijing 100875, China.

The Science of the total environment
|December 31, 2023
PubMed
概括

这项研究完善了一种用于定量污泥中的多酸盐 (多聚) 的方法. 乙烯基二胺三酸 (EDTA) 方法需要对准确性进行调整,考虑到细胞破坏和其他形式.

关键词:
艾迪塔 (EDTA) 是一个叫做EDTA的语言.增强了生物去除的功能.聚酸是一种多酸盐.聚酸积聚生物体量化 量化 量化 量化

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A Practical and Novel Method to Extract Genomic DNA from Blood Collection Kits for Plasma Protein Preservation
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Absolute Quantitation of Inositol Pyrophosphates by Capillary Electrophoresis Electrospray Ionization Mass Spectrometry
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Assaying for Inorganic Polyphosphate in Bacteria
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科学领域:

  • 环境微生物学环境微生物学
  • 生物技术是生物技术.
  • 污水处理 污水处理 污水处理

背景情况:

  • 聚酸盐积聚生物 (PAO) 在废水处理中对生物去除至关重要.
  • 在PAO丰富污泥中定量聚酸盐 (聚P) 对于理解和优化去除过程至关重要.
  • 之前的聚P量化方法,如乙烯基胺三酸 (EDTA) 方法,需要进一步验证和优化.

研究的目的:

  • 重新评估基于EDTA的方法的准确性和特异性,用于量化PAO丰富污泥中的聚P.
  • 在PAO培养物中优化聚聚积累的条件.
  • 确定PAO物种及其在异质化中的作用.

主要方法:

  • 在培养基中优化了N/P比,以促进聚P的形成.
  • 使用光显微镜证实Corynebacterium glutamicum作为一种能够进行异质化PAO物种.
  • 在各种PAO和非PAO生物质样本和污泥上测试了基于EDTA的量化方法.

主要成果:

  • 发现1%的EDTA处理对非PAO细胞具有破坏性,并释放其他形式,影响量化准确性.
  • 应减去大约8毫克Pg-1总悬浮固体的校正因数,以计算非多聚释放的.
  • 释放的量受到PAO分数,社区结构和最初的污泥含量的影响.

结论:

  • 基于EDTA的聚P量化方法需要大幅调整,以获得精确的污泥样本结果.
  • 除了PAO分数之外的因素,包括社区结构和污泥P含量,影响释放动态.
  • 为了可靠地评估废水处理中的PAO活性,需要进一步完善量化方法.