在普通鱼 (Cyprinus carpio) 中的中链脂肪酸受体GPR84的结构和药理特征
Ming-Xing Yao1, Hui-Xia Yu1, Hao-Lin Mo1
1College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, 712100, China.
Developmental and comparative immunology
|December 31, 2023
概括
研究人员在常见鱼中确定了G蛋白结合受体84 (GPR84),主要在肠道和脏中发现. 特定的中链脂肪酸不同调节其信号通路,影响炎症反应.
科学领域:
- *分子生物学和比较基因组学.
- * 鱼类免疫学和生理学.
背景情况:
- *G蛋白结合受体84 (GPR84) 是哺乳动物炎症反应的关键调节者.
- * 它在鱼类中的功能和分布,特别是普通鱼,仍然基本上没有特征.
- *了解鱼类中的GPR84对于研究水产养殖中的炎症过程至关重要.
研究的目的:
- *为了克隆和描述普通鱼GPR84 (ccGPR84).
- * 为了确定ccGPR84在常见鱼的组织分布.
- *通过ccGPR84.4研究由中链脂肪酸 (MCFA) 调节的细胞信号通路.
主要方法:
- * ccGPR84编码序列 (CDS) 的克隆.
- * 用进化背景进行基因组和染色体合成分析.
- *实时定量PCR (qPCR) 用于组织表达分析.
- * 路西法酶记者测定用于评估信号通路激活 (cAMP和MAPK/ERK).
主要成果:
- *ccGPR84 CDS为1191 bp,编码一个396氨基酸蛋白质,在Cyprinids中保存.
- *ccGPR84的表达在肠道和脏中是最高的.
- * 非酸,酸 (二酸),非酸和酸差异性抑制cAMP,并通过ccGPR84激活MAPK/ERK信号.
结论:
- *ccGPR84是常见的鱼中保存的G蛋白结合受体,具有明显的组织表达.
- * 特定的MCFA在调节ccGPR84介导的cAMP和MAPK/ERK信号方面表现出不同的强度.
- *这些发现为了解MCFA在常见鱼炎症反应中的作用提供了基础.
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