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传染体内素对关键的Enterobacteriaceae抗生素耐药细菌的抗菌活性

Tiago Gonçalves¹, Andreia T Marques², Vera Manageiro³

¹Pathogen Genome Bioinformatics and Computational Biology, Research Institute for Medicines (iMed.ULisboa), Faculty of Pharmacy, Universidade de Lisboa, 1649-003 Lisboa, Portugal; Advanced Technologies for Drug Delivery, Research Institute for Medicines (iMed-ULisboa), Faculty of Pharmacy, Universidade de Lisboa, 1649-003 Lisboa, Portugal.

International journal of pharmaceutics

|December 31, 2023

概括

脂质体封装的内素显示出增强的抗菌活性对抗多药耐药的Enterobacteriaceae. 这种新的菌体治疗方法为治疗由优先病原体引起的感染提供了有希望的解决方案.

科学领域:

微生物学微生物学
生物技术是生物技术.
药物运输药物运输药物运输

背景情况:

肠杆菌,包括多药耐药的Klebsiella肺炎,是全球严重的健康威胁.
使用内素的菌体治疗是抗生素治疗细菌感染的潜在替代方案.
格拉姆阴性细菌的外膜对内溶素的输送构成了挑战.

研究的目的:

评估脂质体封装的内素对抗多药耐药的Enterobacteriaceae的疗效.
研究脂质体作为增强内素活性的输送系统的潜力.
评估特定内素的抗菌光谱.

主要方法:

从Enterobacterales物种中净化三种内素 (Kp2948-Lys,Ps3418-Lys,Kaer26608-Lys) 进行净化.
将内素封装到脂质体中 (DMPC:DOPE:CHEMS).
抗菌活性测定和zymogram分析.

主要成果:

纯化内素显示出特定的抗菌活性.
脂质体中的封装效率在24-27%之间.
脂质体封装的内氨酸表现出明显更高的抗菌活性比自由的内氨酸.

结论:

脂质体介导的输送增强了对格拉姆阴性细菌的内素疗效.
Kp2948-Lys对K. pneumoniae表现出特异性;Ps3418-Lys和Kaer26608-Lys具有更广泛的光谱.
在One Health框架内,脂质体封装的内素代表了一种有前途的策略,用于对抗多种耐药性肠杆菌.

关键词:

抗生素耐药性抗生素耐药性恩多利辛 (Endolysin) 是一种内分泌物.它们是Enterobacteriaceae的细菌.克莱布西拉肺炎是一种肺炎.脂质细胞体中的脂肪体.菌体疗法是一种菌体疗法.

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