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从新鲜到腐烂的肉类中微生物群的变化,通过培养和16S rRNA分析确定
Samart Dorn-In1, Sirkka Mang2, Raúl O Cosentino3
1Unit of Food Hygiene and Technology, Institute of Food Safety, Food Technology and Veterinary Public Health, University of Veterinary Medicine, Vienna Veterinaerplatz 1, 1210 Vienna, Austria.
Journal of food protection
|December 31, 2023
概括
新的研究确定了特定的细菌作为肉类卫生和腐败的指标. 这种"卫生指数"有助于评估肉类的质量和安全性,即使在加工产品中也是如此,从而提高了食品的安全性.
科学领域:
- 食品微生物学 食品微生物学
- 微生物生态学 微生物生态学
- 食品安全 食品安全
背景情况:
- 肉类腐烂通常与微生物生长有关,但高细菌负载并不总是与感官变化相关.
- 评估肉类的真正卫生状况和安全性需要超出感官感知范围的可靠指标.
研究的目的:
- 为了研究在不同温度下储存肉类微生物群的变化.
- 为了确定特定的细菌物种作为肉类卫生和腐败的指标.
- 开发一个"卫生指数"来评估肉类的质量和安全.
主要方法:
- 使用下一代测序 (NGS) 和盘子计数分析猪肉微生物群.
- 猪肉样本在4°C和22°C的温度下在不同时间段储存.
- 根据卫生与腐烂指标细菌的比例计算"卫生指数".
主要成果:
- 伪omonas spp. 这种类型的. 和乳酸细菌 (LAB) 被确定为关键的"破坏指标细菌".
- 斯塔菲洛科克斯 (Staphylococcus epidermidis) 被确定为一种在新鲜肉中普遍存在的"卫生指标细菌".
- "卫生指数"显示与细菌负载有显著的相关性,表明其实用性.
结论:
- 通过NGS确定特定细菌的比例,可以有效地表明肉类的卫生状况.
- 开发的"卫生指数"为评估肉类质量和安全提供了一种新的方法.
- 这种方法提高了食品质量保证和消费者保护,适用于加工肉制品.
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