长期携带和进化VREfm长期携带和进化抗万科素Enterococcus faecium:一系列分离的基因组研究
Maja Johanne Søndergaard Knudsen1, Jose Alfredo Samaniego Castruita1, Sarah Mollerup1
1Department of Clinical Microbiology, Copenhagen University Hospital-Amager and Hvidovre, Hvidovre, Denmark.
JAC-antimicrobial resistance
|January 1, 2024
概括
长期耐旺素的Enterococcus faecium (VREfm) 携带者通常保持相同的VREfm克隆. 基因组分析显示,在这些持久的VREfm感染中,每年平均每基因组发生2.6个单核酸多态 (SNP).
科学领域:
- 传染性疾病 传染性疾病
- 基因组学就是基因组学.
- 微生物学 微生物学
背景情况:
- 抗万科米辛的Enterococcus faecium (VREfm) 是一个重要的与医疗相关的病原体.
- 了解VREfm克隆在携带者中的持久性和演变对于感染控制至关重要.
研究的目的:
- 为了确定是否具有抗胺素的Enterococcus faecium (VREfm) 携带者至少在365天后都携带相同的VREfm克隆.
- 在长期载体中研究VREfm克隆的基因组进化,以每年,每基因组为基础.
主要方法:
- 整个基因组测序 (WGS) 用于识别和分配VREfm克隆到分离物.
- 克隆转移是通过比较来自顺序分离的WGS数据来确定的.
- 核基因组多部位序列类型化 (MLST) 的等位基因距离和单核酸多态性 (SNP) 计数计算在同一克隆的连续分离物之间.
主要成果:
- 在整个研究期间,很大一部分患者 (44.2%) 携带相同的VREfm克隆.
- 基因组进化率量化为每年每基因组1.8个核心基因组MLST基因和2.6个SNP.
- 这些数据建立了长期载体中VREfm进化的分子钟.
结论:
- 长期的VREfm携带通常以单个克隆的持久性为特征.
- 计算的基因组进化率为VREfm的分子钟提供了洞察力.
- 这些发现对理解VREfm传输动态和制定有针对性的干预措施有影响.
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