[肺结节患者唾液微生物群特征分析:前性非随机并发受控试验]
Yifeng Ren1,2, Qiong Ma1, Fang Li1
1（ 610075） Sichuan Provincial Key Laboratory of TCM Regulation of Metabolic Diseases, Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China.
概括
唾液微生物群在患有肺结节 (PN) 的患者中显著不同. 像Porphyromonas,Haemophilus和Fusobacterium这样的特定细菌显示出作为早期PN检测的非侵入性生物标志物的潜力.
科学领域:
- 微生物学 微生物学
- 肺部医学 肺部医学
- 生物标志物发现发现
背景情况:
- 肺结节 (PN) 需要准确的早期检测方法.
- 唾液微生物群的改变可能与各种疾病有关.
- 了解这些变化可能会为诊断提供新的途径.
研究的目的:
- 为了研究PN患者和健康对照者之间的唾液微生物群的差异.
- 为了确定潜在的唾液微生物生物标志物用于早期PN检测.
主要方法:
- 来自173名PN患者和40名对照患者的唾液的16S rRNA扩增序列测序.
- 微生物多样性,组成和差异性物种的分析.
- 随机森林算法用于生物标志物识别和预测价值评估 (AUC).
- PICRUSt2用于微生物群落的功能概况.
主要成果:
- 与对照组相比,PN患者的唾液微生物群的α和β多样性增加 (P<0.05).
- 微生物群落组成和丰富度的显著差异.
- 鉴定出Porphyromonas,Haemophilus和Fusobacterium是最优的PN生物标志物 (AUC=0.79) 的发现.
- 丰富PN唾液微生物群中的免疫缺陷和氧化还原平衡功能.
结论:
- 在患有肺结节的个体中,唾液微生物群的组成发生了显著的改变.
- 特定的细菌物种显示为早期PN诊断的非侵入性生物标志物具有前途.
- 唾液微生物群可能在PN发展或恶性转变中发挥作用.
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