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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

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相关实验视频

Updated: Jul 6, 2025

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

带有单分子敏感性的键选择性光成像.

Haomin Wang¹, Dongkwan Lee¹, Yulu Cao¹

¹Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, USA.

Nature photonics

|January 1, 2024

概括

此摘要是机器生成的。

我们开发了Bond选择性光检测红外激发 (BonFIRE) 光谱显微镜,用于敏感的生物成像. 这种技术在具有单分子敏感性的细胞和组织中提供特定键的化学信息.

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相关实验视频

Last Updated: Jul 6, 2025

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Published on: October 28, 2018

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules

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Published on: November 2, 2009

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科学领域:

光学成像技术的使用.
振动光谱学是一种振动光谱法.
分子生物学分子生物学

背景情况:

生物成像需要高化学特异性和灵敏度.
中红外 (中红外) 振动光谱提供了丰富的化学信息,但缺乏生物成像的灵敏度.
现有的方法很难为复杂的生物系统提供详细的分子见解.

研究的目的:

开发一种高度敏感的生物成像技术,用于键选择性化学分析.
为了克服生物应用中等红外振动光谱的灵敏度限制.
为了能够在细胞,神经元和组织中进行详细的分子询问.

主要方法:

开发了带有选择性的光检测红外激发 (BonFIRE) 光谱显微镜.
利用中红外和近红外的两光子激发来向上转换振动激发.
采用可调节的窄带皮秒脉冲,以提高灵敏度和生物相容性.
在指纹和细胞静音窗口中展示了光谱成像,具有单分子灵敏度.

主要成果:

使用BonFIRE实现了对光染料的单分子敏感性.
在固定和活细胞,神经元和组织中成功成像细胞内目标.
使用高频调制证明了活HeLa细胞的BonFIRE时间缩短显微镜.
展示了振动多重复合用于高级分析的潜力.

结论:

邦菲尔光谱显微镜显著提高了对振动生物成像的灵敏度.
这项技术提供了前所未有的键-特定的分子信息.
BonFIRE扩展了生物成像工具箱,用于生物研究中的功能成像和传感.