相关实验视频

Updated: Jul 6, 2025

Vasodilation of Isolated Vessels and the Isolation of the Extracellular Matrix of Tight-skin Mice

Vasodilation of Isolated Vessels and the Isolation of the Extracellular Matrix of Tight-skin Mice

Published on: March 24, 2017

I型干扰素在系统性红斑狼中以Tie2依赖的方式诱导内皮质不稳定

Carlos Rafael-Vidal^1,2, Sara Martínez-Ramos^1,2, Beatriz Malvar-Fernández^1,2

¹Rheumatology and Immune-mediated Diseases Group, Galicia Sur Health Research Institute (IIS Galicia Sur), Vigo, Spain.

Frontiers in immunology

|January 1, 2024

概括

I型干扰素 (IFN) 通过抑制Tie2信号来损害系统性红斑狼 (SLE) 的血管稳定性,导致内皮细胞功能障碍和潜在的心血管事件.

科学领域:

免疫学免疫学免疫学
血管生物学血管生物学
类风湿病学类风湿病学

背景情况:

内皮细胞 (EC) 功能障碍和Tie2受体信号传递在系统性红斑狼 (SLE) 病原发生过程中至关重要.
炎症过程,特别是I型干扰素 (IFN),与SLE的Tie2通路失调和血管并发症有关.

研究的目的:

调查I型IFN在SLE患者中介于Tie2信号诱导的内皮功能障碍中的作用.
评估I型IFN和SLE血清对angiopoietin-Tie2轴组件和EC功能的影响.

主要方法:

在SLE患者和对照人群中测量了Angiopoietin-1,Angiopoietin-2和溶性Tie1的血清水平.
单细胞和人静脉ECs (HUVECs) 被I型IFN或SLE血清刺激.
使用qPCR,ELISA,西布洛特,流细胞计,共聚焦显微镜,素和管形成试验,分析了mRNA/蛋白质表达,酸化,转位,EC活力和血管生成.

主要成果:

患有SLE的患者表现出升高的sTie1和Ang-2,以及降低的Ang-1,与临床特征相关.
I型IFN降低了SLE单细胞中的Ang-1,增加了SLE单细胞中的Ang-2,增加了sTie1和Ang-2分泌,同时降低了HUVECs中的Tie2激活.
I型IFN以Tie2依赖的方式损害了EC活力和血管生成;SLE血清模仿了这些效应,这些效应被Tie1和IFNAR1敲击扭转.

结论:

I型IFN通过抑制Tie2信号传递,有助于导致SLE内皮细胞不稳定.
这些发现表明,一种机制将I型IFN介导的内皮功能障碍与SLE中心血管事件联系在一起.

关键词:

这是一张领带.血管新生素是一种血管新生素.心血管疾病的风险.内皮细胞的破坏稳定系统性红血性狼 (Systemic Lupus Erythematosus) 是一种全身性狼.第一种类型的干扰子干扰子.

更多相关视频

Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α

Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α

Published on: June 14, 2018

Native Polyacrylamide Gel Electrophoresis Immunoblot Analysis of Endogenous IRF5 Dimerization

Native Polyacrylamide Gel Electrophoresis Immunoblot Analysis of Endogenous IRF5 Dimerization

Published on: October 6, 2019

相关概念视频

T Cell Types and Functions

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...

Intracellular Signaling Affects Focal Adhesions

Intracellular Signaling Affects Focal Adhesions

Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...

Regulation of Angiogenesis and Blood Supply

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...