物理和抗氧化剂反应与Sub1基因与完全潜水下的米 (Oryza sativa L.) 线相关
1Department of Biotechnology, The Cochin College, Kochi, 682002 India.
概括
研究人员使用标记器辅助逆交繁殖将潜水耐受性 (Sub1) 基因引入了贾亚大米品种. 新的 Sub1 Jaya 线路显示,在洪水下生存能力有所提高,这对于易受洪水袭击的地区至关重要.
科学领域:
- 植物育种和遗传学 植物育种和遗传学
- 农作物科学 农作物科学
- 农业学是一种农业学.
背景情况:
- 米种植受到淹水的重大影响,特别是在易受洪水袭击的地区.
- Sub1基因赋予了对洪水的耐受性,但其侵入精英品种是必不可少的.
- 标记器辅助逆交繁殖 (MABB) 是提高作物的关键策略.
研究的目的:
- 用MABB.将潜水耐受性 (Sub1) 基因侵入精英大米品种Jaya,使用MABB.
- 为了评估潜水下的Sub1-introgressed线的生理和生化反应.
- 为受洪水影响地区的农民开发耐浸水的米品种.
主要方法:
- 标记器辅助逆交繁殖 (MABB) 用于将Sub1基因侵入贾亚大米.
- 20个Sub1内进线的发展,具有定义的Sub1区域 (3.1-5.1Mb) 和高复发性父基因组恢复 (80.0-95.3%).
- 生理和生化评估包括生存百分比,水下芽延长,碳水化合物含量和超氧化物脱酶活性.
主要成果:
- 成功开发了20个Sub1内进的Jaya线,具有显著的重复性父基因组恢复.
- 亚1内进线表现出减少的水下射线延长和显著更高的超氧化物脱酶活性 (约. 5倍) 在潜水后.
- 这些生理改善导致Sub1内进线的生存率高于对照线.
结论:
- 标记器辅助的Sub1基因进入贾亚大米成功实现了.
- 开发的Sub1 Jaya线路显示了通过生理标志物验证的增强的潜水耐受性.
- 这些线条有可能作为一种新的大米品种释放,有利于喀拉拉邦等易受洪水影响的地区的农民.
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