一个基于FRET的比度H2S传感器用于在第二近红外窗口中的敏感光学分子成像
Shan Lei1, Kejia Jiang1, Chenqing Zhang1
1Marshall Laboratory of Biomedical Engineering, International Cancer Center, Shenzhen Key Laboratory of Tumor Visualization Molecular Medicine, Laboratory of Evolutionary Theranostics (LET), School of Biomedical Engineering, Shenzhen University Medical School, Shenzhen University, Shenzhen 518055, China.
Research (Washington, D.C.)
|January 1, 2024
概括
研究人员开发了一种用于在第二次近红外 (NIR-II) 窗口中检测硫化 (H2S) 的新型比度传感器. 这种先进的成像工具为体内瘤成像和药物效应监测提供了高灵敏度和选择性.
科学领域:
- 生物医学成像技术 生物医学成像技术
- 分子成像学分子成像学
- 光学成像技术的成像
背景情况:
- 第二次近红外 (NIR-II) 窗口成像提供高分辨率的体内可视化.
- 挑战包括显著的自光背景和NIR-II应用的有限探头开发.
研究的目的:
- 在NIR-II窗口中开发用于硫化 (H2S) 检测的比度传感器.
- 为了使H2S水平的敏感和选择性体内成像能够用于诊断目的.
主要方法:
- 构建了一个基于Förster共振能量转移 (FRET) 的传感器,结合了H2S敏感的氨酸染料 (LET-1055) 和H2S惰性罗达胺染料 (Rh930).
- 评价传感器性能,包括灵敏度,选择性,反应动力学和H2S检测极限.
主要成果:
- 开发的传感器表现出对H2S的高灵敏度和选择性.
- 实现了快速反应动力学 (~20分钟),具有较低的检测极限 (~96nM).
- 成功应用传感器用于肝脏和结肠瘤的体内比度NIR-II光成像,并可视化肝脏中药物诱导的H2S波动.
结论:
- 基于FRET的比度NIR-II传感器为基于H2S活动的传感提供了一个强大的工具.
- 这项技术在推进体内临床诊断和监测治疗反应方面具有重大潜力.
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