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用HMO为原料的双细菌表现出增强粘附于肠道上皮细胞的能力

Clodagh Walsh^1,2,3, Rebecca A Owens⁴, Francesca Bottacini^3,5

¹Teagasc Food Research Centre, Moorepark, Cork, Ireland.

Frontiers in microbiology

|January 1, 2024

概括

人类牛奶寡糖 (HMOs) 显著增加有益的Bifidobacteria对肠道细胞的粘附. 这种原始效应,特别是多菌株组合,增强了益生菌的殖民和潜在的健康益处.

科学领域:

微生物学微生物学
胃肠病学胃肠病学
营养科学营养科学

背景情况:

肠道共生体对肠道上皮质的粘附影响了微生物群的组成.
广泛使用的益生菌Bifidobacteria提供了许多健康益处.
增强的双菌粘附可以放大宿主-微生物相互作用,从而获得更大的健康优势.

研究的目的:

为了研究母乳寡糖 (HMO) 在体外改善双细菌肠粘附的能力.
评估HMO对婴儿相关的Bifidobacterium菌株的殖民促进作用.

主要方法:

使用HT29-MTX细胞作为体外肠道模型进行细菌粘附测试.
暴露了四种与婴儿相关的商业Bifidobacterium菌株 (B. longum亚种. 婴儿,B. 简短,B. 两) 到HMO.
对暴露于HMO的多菌株双菌混合物进行蛋白质组分析.

主要成果:

短期暴露于HMO增加了单个Bifidobacterium菌株的粘附率高达47%.
用HMOs作为四个菌株组合的化导致粘附活性的细菌增加了90%以上.
蛋白质组分析表明,B. bifidum中依赖于酸酶的 pili 和 glycosyl 酸酶的丰度增加.

结论:

HMO可以显著增强婴儿肠关联的Bifidobacterium与肠上皮细胞的附着性.
通过调节粘附因子的表达,HMOs可以通过调节粘附因子来培养Bifidobacterium的殖民地.
这种由HMO调解的增强粘附机制有可能提高益生菌的疗效.

关键词:

这种细菌是Bifidobacterium.这是一个HMO.母乳母乳是母乳中的一种.殖民化殖民化的殖民化这就是HIEECs.益生菌益生菌益生菌益生菌是一种益生菌.

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