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在ZmKW1中的遗传变异有助于玉米和爆米花中核的重量和大小

Long Zhang^1,2, Miaomiao Fu³, Wenyu Li¹

¹State Key Laboratory of Wheat and Maize Crop Science, Collaborative Innovation Center of Henan Grain Crops, College of Agronomy, Henan Agricultural University, Zhengzhou, China.

Plant biotechnology journal

|January 1, 2024

概括

一个主要的基因,ZmKW1,控制着玉米核的重量和大小. 它的促进活性中的差异解释了为什么爆米花核比角玉米核要小,从而影响了产量.

科学领域:

植物遗传学植物遗传学
玉米育种玉米育种玉米育种
分子生物学分子生物学

背景情况:

玉米核的重量对于玉米 (Zea mays) 的产量至关重要.
由于遗传因素,爆米花核自然比角玉米核小.
定量特征位置 (QTL) 影响核的重量和尺寸变化.

研究的目的:

识别和描述玉米中核重量和大小变化的遗传基础.
了解1号染色体上主要QTL,qKW1的分子机制.
研究ZmKW1在调节内发育和核特征中的作用.

主要方法:

定量特征位置 (QTL) 映射和表征.
基因编辑 (CRISPR-Cas9) 用于研究ZmKW1在牙玉米中的功能.
分析ZmKW1表达和蛋白质活性.
促进体分析和链接不平衡 (LD) 研究.

主要成果:

一个主要的QTL,qKW1,在染色体1上显著控制玉米核的重量和大小.
ZmKW1编码了一个E3泛素酶,通过影响内精细胞数量,大小和填充,负面调节核重量和大小.
基因编辑证实了ZmKW1的作用:突变增加了内核重量,而过度表达减少了它.
爆米花和角玉米中相同的ZmKW1蛋白质,但爆米花中的促进子插入 (Indel-1346) 会导致更高的表达和更小的核.
在LD分析中,ZmKW1促销器区域中发现了两种不同的单元类型.

结论:

ZmKW1是玉米核重量和大小的关键负调节器.
由Indel-1346插入驱动的ZmKW1促进体活性的变化解释了爆米花和角玉米核大小之间的差异.
无处不在的系统在调节玉米核的发育和玉米品种的产量方面发挥着重要作用.

关键词:

齐亚梅斯 (Zea mays) 是一种植物.内精细胞的细胞发育.核的重量和大小核的重量和大小爆米花是因为爆米花.无处不在的化途径

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