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使用先进的VOC分析技术进行非破坏性卵种分离 HSSE-GC-MS,PTR-TOF-MS和SIFT-MS:性能评估和系统的互补性

Matthias Corion¹, Miguel Portillo-Estrada², Simão Santos¹

¹KU Leuven, BIOSYST-MeBioS Biosensors Group, Department of Biosystems, Leuven, Belgium.

Food research international (Ottawa, Ont.)

|January 1, 2024

概括

蛋中的挥发性有机化合物 (VOC) 可以揭示品种差异. 三种质谱技术有效地确定了VOC,使得高精度的非破坏性品种歧视成为可能.

科学领域:

分析化学分析化学
农业科学农业科学
生物化学生物化学

背景情况:

蛋中的挥发性有机化合物 (VOC) 提供了关于新鲜度,生育能力,裂,胚胎性别和品种的见解.
对于检查这些VOC来说,先进的分析技术至关重要.

研究的目的:

评估三种基于质谱的系统,用于分析卵中的VOC.
为了识别可区分三种不同的品种的VOC,而不论化日.

主要方法:

评估的头空吸收提取气体染色体质谱 (HSSE-GC-MS),质子转移反应飞行时间质谱 (PTR-TOF-MS) 和选择的离子流管质谱 (SIFT-MS).
分析了Dekalb白层,Shaver棕层和Ross 308肉品种在第2和第8个化日的完整的卵.
使用部分最小平方差分分析 (PLS-DA) 进行品种差异化和变量选择.

主要成果:

使用HSSE-GC-MS识别了111种挥发性有机化合物,其中酒精和醇是最丰富的.
PLS-DA模型显示了高品种歧视效率:HSSE-GC-MS (95.5%的准确率) 和PTR-TOF-MS (95.5%的准确率).
观察到HSSE-GC-MS (高分子量VOC) 和PTR-TOF-MS/SIFT-MS之间的互补性,其中PTR-TOF-MS显示出更好的量化相关性.

结论:

挥发性有机化合物分析系统可以非破坏性地识别卵中的品种特异性差异.
该研究强调了VOC分析在质量控制和揭示生物信息或管理实践变化的潜力.
通过PTR-TOF-MS证明了通过HSSE-GC-MS识别的特定挥发性有机化合物的量化有很大的潜力.

关键词:

卵品种歧视卵品种歧视这就是HSSE-GC-MS.卵是卵中的一个.质谱测量质量谱测量这是一个PTR-TOF-MS.这就是SIFT-MS.挥发性有机化合物分析

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