来自酵母精密发酵的食品蛋白质:使用聚酸盐纯净复合β-乳球蛋白的简单净化
L J G Hoppenreijs1, A Annibal2, G J C Vreeke3
1Laboratory of Food Process Engineering, Wageningen University & Research, Bornse Weilanden 9, 6708 WG Wageningen, the Netherlands.
Food research international (Ottawa, Ont.)
|January 1, 2024
概括
一种新的,具有成本效益的方法使用六甲酸盐 (HMP) 净化酵母产生的蛋白质. 这种简单的沉技术可以提高蛋白质的纯度,而不会影响其结构或功能,为食品应用提供了可行的染色学替代方案.
科学领域:
- 生物技术是生物技术.
- 食品科学 食品科学 食品科学
- 生物化学 生物化学
背景情况:
- 精密发酵产生需要净化的蛋白质,通常是通过染色学.
- 目前的净化方法可能会缓慢而昂贵,特别是在食品应用中.
- 需要有效和经济的蛋白质净化策略.
研究的目的:
- 为酵母表达的蛋白质开发一种简单,具有成本效益的净化方法.
- 为了评估六甲酸盐 (HMP) 沉用于蛋白质净化的有效性.
- 评估净化方法对蛋白质结构和功能的影响.
主要方法:
- 使用食品级六甲酸盐 (HMP) 在酸性pH下进行蛋白质沉.
- 通过中和重新溶解了蛋白质-HMP复合物.
- 使用化沉分离过剩的HMP.
- 来自Pichia pastoris的纯化β-乳球蛋白作为一种模型蛋白质.
主要成果:
- 蛋白质含量从26 wt%增加到72 wt%,可与离子交换染色学相比较.
- 杂质减少到9重%的细胞外多糖和1重%的HMP.
- 保持净化蛋白质的结构和功能特性,包括乳液形成.
- 证明了目标蛋白质与酵母衍生的多糖的有效分离.
结论:
- 六甲酸盐 (HMP) 沉为酵母产生的蛋白质提供了一种简单有效的净化方法.
- 这种方法具有成本效益,适合食品应用.
- 该技术保持了蛋白质的完整性和功能性,为传统染色学提供了一个有希望的替代方案.
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