制备,结构特征和性质的铁醇寡糖 - 米蛋白水解合物合物
Fenghong Deng1, Shunjing Luo1, Xiuting Hu1
1The State Key Laboratory of Food Science and Resources, Nanchang University, Nanchang 330047, Jiangxi, China; International Institute of Food Innovation Co. Ltd, Nanchang 330200, Jiangxi, China.
Food research international (Ottawa, Ont.)
|January 1, 2024
概括
米蛋白水解盐 (RPH) 与铁醇寡糖 (FO) 相结合,增强了溶解性和抗氧化活性. 不同的结合方法产生了不同程度的修饰,影响了RPH的特性,如乳化.
科学领域:
- 食品科学 食品科学 食品科学
- 生物化学 生化学
- 蛋白质化学 蛋白质化学
背景情况:
- 米蛋白水解盐 (RPH) 是生物活性的宝贵来源.
- 铁醇寡糖 (FOs) 是具有抗氧化特性的功能性碳水化合物.
- 结合可以改变蛋白质和碳水化合物的功能性质.
研究的目的:
- 使用乳糖酶和自由基催化剂将RPH与FO结合在一起.
- 为了研究由此产生的结合物的结构和性质变化.
- 为了比较不同合方法对RPH-FO合物的影响.
主要方法:
- 酶 (laccase) 和自由基催化RPH和FO的结合.
- 用于分子量分析的电泳.
- 用于结构分析的光谱技术 (紫外线,光,圆形二重化).
- 测试可溶性,抗氧化活性,乳化活性和稳定性.
主要成果:
- 通过增加分子量证实了RPH-FO合物的成功形成.
- 乳糖酶结合主要涉及氨酸残留物,而基结结合涉及氨基和氨酸组.
- 结合改变了RPH的二级和三级结构,增加了可溶性和抗氧化活性,但降低了乳化特性.
- 与激素诱导的合物相比,激素诱导的合物具有较高的抗聚合和抗氧化能力,但乳化活性较低.
结论:
- 与FO的结合会改变RPH结构和功能性质.
- 选择的结合方法 (laccase与自由基) 显著影响结合的程度和由此产生的特性.
- RPH-FO结合物显示出需要增强抗氧化活性和可溶性应用的潜力,在乳化方面有权衡.
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