PatWRKY71转录因子调节了patchoulol生物合成和植物防御反应
Jian Li1,2, Huan-Chao Huang3, Yue-Qiu Zuo1,2
1State Key Laboratory of Plant Diversity and Specialty Crops, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China.
BMC plant biology
|January 2, 2024
概括
酸 (SA) 通过上调转录因子 PatWRKY71.7 的调节,增强了 Pogostemon cablin 中的 patchoulol 生产. 这一发现为通过基因工程改善包醇含量和植物耐药性的途径提供了途径.
科学领域:
- 植物分子生物学 植物分子生物学
- 植物生物化学 植物生物化学
- 代谢工程是代谢工程.
背景情况:
- 帕丘,帕丘油中的一个关键的基基,具有重要的医疗和化品应用.
- 帕丘洛尔生物合成的转录调节仍未得到充分研究.
- 酸 (SA) 是一种关键的植物激素,参与生长和防御.
研究的目的:
- 为了研究酸 (SA) 对Pogostemon cablin (P. cablin) 的patchoulol生物合成的影响.
- 为了确定涉及SA介导的patchoulol生产的关键监管因素.
- 阐明已识别的因素在patchoulol生物合成和植物防御中的作用.
主要方法:
- 用SA处理的P. cablin. 的转录组分析.
- 转录因子PatWRKY71.1.的识别和表征
- 对PatWRKY71与PatPTS促进者结合的分析.
- 基因沉默实验和Arabidopsis中的异质表达.
主要成果:
- 在P. cablin. 中,SA治疗显著增加了patchoulol的产量.
- 在SA上调节了转录因子PatWRKY71.1.的表达.
- PatWRKY71直接与PatPTS促进体结合,增强其表达和随后的patchoulol生物合成.
- 沉默PatWRKY71减少了patchoulol的产生.
- 在Arabidopsis中,PatWRKY71的异质表达增加了对盐和压力的敏感性,以及ROS的产生.
结论:
- 酸通过转录因子PatWRKY71.71积极调节P. cablin中的patchoulol生物合成.
- PatWRKY71在帕丘洛尔的生产和植物防御机制中起到关键的调节作用.
- 这项研究为通过基因工程增强帕丘醇含量和P. cablin耐药性的基础.
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