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ZmC2H2-149通过抑制玉米中的ZmHSD1来负面调节干旱耐受性

Huafeng Liu¹, Zhendong Wu¹, Miaomiao Bao¹

¹College of Agronomy, National Key Laboratory of Wheat and Maize Crop Science and Key Laboratory of Regulating and Controlling Crop Growth and Development Ministry of Education, Henan Agricultural University, Zhengzhou, Henan, China.

Plant, cell & environment

|January 2, 2024

概括

科学家们确定了一种玉米基因,ZmC2H2-149,通过抑制ZmHSD1.1来调节干旱耐受性. 这一发现提供了基因资源,用于提高玉米在育种计划中的抗旱能力.

科学领域:

植物生物学植物生物学
遗传学遗传学是一个
农业科学农业科学

背景情况:

干旱压力显著限制了全球玉米产量,需要制定提高作物弹性战略.
已知Cys2/His2指蛋白 (C2H2-ZFPs) 在植物应激耐受性中起作用.

研究的目的:

调查C2H2-ZFPs在玉米干旱耐受性的作用.
确定参与调节玉米对干旱压力的反应的特定基因.

主要方法:

在干旱条件下选150 Ac/Ds突变玉米线.
基因表达分析使用DAP-Seq,RNA-Seq,酵母单杂交 (Y1H) 和露西法酶 (LUC) 试验.
在缺水条件下对突变系 (zmc2h2-149和zmhsd1) 的表型评估.

主要成果:

一个Ds插入突变体,zmc2h2-149,表现出增强的干旱耐受性.
过度表达ZmC2H2-149降低了干旱耐受性和作物产量.
发现ZmC2H2-149直接抑制ZmHSD1的表达,ZmC2H2-149是干旱耐受性的积极调节者.
zmhsd1突变显示干旱耐受性降低和谷物产量.

结论:

通过抑制ZmHSD1.1,ZmC2H2-149对玉米的干旱应激耐受性进行负面调节.
了解ZmC2H2-149/ZmHSD1通路为开发抗旱玉米品种提供了宝贵的见解.
这项研究为玉米育种计划提供了关键的遗传资源,重点是改善干旱耐受性.

关键词:

C2H2 指蛋白质指蛋白质齐亚梅斯 (Zea mays) 是一种植物.干旱造成的压力是干旱.氧类固醇脱酶1 (HSD1) 是一种氧类固醇脱酶.

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