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通过DREB/COR信号通路,ERF54调节了Rosa multiflora中的耐寒性

Linmei Chen¹, Yeni Chen¹, Huanyu Zhang¹

¹Discipline of Ornamental Horticulture, Key Laboratory of Quality and Safety Control for Subtropical Fruit and Vegetable, Ministry of Agriculture and Rural Affairs, Collaborative Innovation Center for Efficient and Green Production of Agriculture in Mountainous Areas of Zhejiang Province, College of Horticulture Science, Zhejiang A & F University, Hangzhou, Zhejiang, China.

Plant, cell & environment

|January 2, 2024

概括

乙烯反应因子 (ERF) 调节寒冷耐受性. 过度表达RmERF54通过激活应激反应基因和抗氧化剂系统,增强了植物的抗寒能力.

科学领域:

植物生物学植物生物学
分子遗传学分子遗传学
压力生理学压力生理学

背景情况:

乙烯反应因子 (ERF) 是植物发育和应激反应的关键调节者.
许多ERF在冷应力耐受性方面的特定作用在很大程度上仍未被描述.

研究的目的:

为了研究RmERF54在Rosa multiflora的寒冷应激反应中的功能.
阐明RmERF54介导的寒冷耐受性背后的分子机制.

主要方法:

在转基因烟草和体胚胎中,RmERF54的过度表达和病毒诱导的基因沉默 (VIGS).
转录组分析以确定下游目标.
电泳运动转移试验 (EMSA) 验证促进体结合.
测量抗氧化酶活性和活性氧物种 (ROS) 水平.

主要成果:

过度表达RmERF54显著增强了转基因植物的抗寒能力.
通过VIGS调节RmERF54的沉默增加了感冒易感性.
RmERF54直接激活了对寒冷反应基因的表达,包括RmPODP7和RmCOR47.
过度表达RmERF54导致抗氧化酶活性增加并降低ROS水平.

结论:

RmERF54在赋予植物耐寒性方面发挥着至关重要的作用.
它作为核转录因子起作用,调节应激反应基因和抗氧化途径的网络.
RmERF54是RmDREB1E的下游目标,为冷应力信号级联提供了洞察力.

关键词:

在 DREB/COR 信号通道上.在RmDREB1E.RmERF54 在线阅读罗莎是多种多样的植物.

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