通过MdASMT9-介导的黑激素生物合成增强了果植物的基底热耐受性
Tengteng Gao1, Danni Zhang1, Wentao Shen1
1State Key Laboratory for Crop Stress Resistance and High-Efficiency Production/Shaanxi Key Laboratory of Apple, Pomology Department, College of Horticulture, Northwest A&F University, Yangling, Shaanxi, China.
Plant, cell & environment
|January 2, 2024
概括
黑素 (MT) 通过增加抗氧化活性和减少压力激素,增强果树的耐热性. 在转基因果中过度表达MT生物合成基因可以提高它们对高温的抗性.
科学领域:
- 植物科学 植物科学
- 分子生物学分子生物学
- 农业 农业 农业 农业
背景情况:
- 高温降低了水果作物的产量和质量.
- 黑素 (MT) 应用改善了植物的耐热性,但其在热应激下多年生果树中的内源性作用尚不清楚.
研究的目的:
- 研究高温对转基因果植物过度表达黑激素生物合成基因MdASMT9.9的影响.
- 阐明内源性黑激素在增强多年果树耐热性中的作用.
主要方法:
- 产生和分析过度表达MdASMT9.9的转基因果植物.
- 测量内源性黑激素水平,抗氧化酶活性,活性氧物种 (ROS) 清除,以及在热应激下叶绿体的完整性.
- 对酸 (ABA) 积累,口腔孔口和自活动的分析.
- 关键调节基因 (MdWRKY33,MdNCED1,MdNCED3,MdATG18a) 的基因表达分析.
主要成果:
- 转基因果植物中的内源性黑激素通过增加抗氧化活性,清除ROS,并保存叶绿体,从而保护其免受热应激.
- 黑素的应用和MdASMT9的过度表达减少了酸的积累,通过MdWRKY33介导的MdNCED1/3.3的抑制通过MdWRKY33介导的MdNCED1/3.3的抑制促进热散发的口腔开口.
- 黑色素通过在热应激下通过MdWRKY33介导的MdATG18a的上调来增强自活动.
结论:
- 内源性黑激素在赋予多年生果树基本耐热性方面发挥着至关重要的作用.
- 黑色素调节抗氧化剂防御,口腔功能和自通路,以减轻热应激损伤.
- 研究结果提供了对内源性黑色素调节及其改善作物耐热性潜力的新见解.
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