通过ALYREF介导的RNA5-甲基细胞素修饰 通过稳定EGFR mRNA和pSTAT3激活促进肝细胞癌的进展
Jiayida Nulali1, Kaiwen Zhang1, Manmei Long2
1The Core Laboratory in Medical Center of Clinical Research, Department of Molecular Diagnostics and Endocrinology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China.
International journal of biological sciences
|January 2, 2024
概括
艾利/REF出口因子 (ALYREF) 通过稳定EGFRmRNA来促进肝癌. 这种m5C阅读器在LIHC中升高,通过EGFR和STAT3通路驱动扩散,入侵和不良预后.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 在癌症中,5-甲基细胞氨酸 (m5C) 的mRNA修饰至关重要.
- 艾利/REF出口因子 (ALYREF) 与肝肝细胞癌 (LIHC) 的预后有关.
- 艾利瑞夫在LIHC进展中的确切作用及其机制尚不清楚.
研究的目的:
- 研究ALYREF在LIHC进展中的功能作用.
- 阐明ALYREF在LIHC中的作用背后的分子机制.
- 确定ALYREF与临床病理学参数和患者预后的关联.
主要方法:
- 分析在线数据库和独立的LIHC队列.
- 在体外实验评估细胞增殖,迁移,入侵和EMT.
- 在体内瘤形成测试.
- 涉及EGFR通路,m5C修饰部位和STAT3信号的机制研究.
主要成果:
- 在LIHC组织中,ALYREF显著升高,与预后不佳,高度TNM阶段和Ki67+细胞增加相关.
- 艾利瑞夫可以增强LIHC细胞的增殖,迁移,入侵,EMT和瘤生长 in vivo.
- ALYREF通过结合其在3' UTR中的m5C修饰位来稳定EGFRmRNA,从而激活STAT3通路.
结论:
- 艾利瑞夫作为LIHC的关键瘤原因子.
- 通过稳定EGFRmRNA和激活STAT3信号,ALYREF促进LIHC的进展.
- 了解ALYREF的作用为LIHC提供了潜在的治疗点.
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