接收氨酸的tRNA类结构的多样性和模块化
Madeline E Sherlock1,2, Conner J Langeberg1, Jeffrey S Kieft3,2
1Department of Biochemistry and Molecular Genetics, University of Colorado Anschutz Medical Campus, Aurora, Colorado 80045, USA.
概括
病毒RNA结构称为tRNA样结构 (TLSs),接受氨酸,与病毒相比,在群体病毒中具有不同的5'域. 这种结构差异对于高效的氨基化至关重要,证明了病毒RNA进化的模块性.
科学领域:
- 病毒学 病毒学
- 分子生物学分子生物学
- 生物信息学是一种生物信息学.
背景情况:
- 阳性感单链RNA病毒具有3'tRNA样结构 (TLSs) 通过宿主酶进行氨基化.
- 来自不同病毒家族的氨酸接受TLS (TLSTyr) 在它们的结构中表现出差异,特别是在负责抗模仿的5'域中.
研究的目的:
- 为了研究TLSTyr 5'域的结构和功能上的差异,在病毒在hordeivirus和Bromoviridae家族.
- 为了确定5'域在hordeivirus TLSTyr的氨基化效率中的作用.
- 探索不同TLS结构之间的进化关系.
主要方法:
- 病毒RNA二次结构的生物信息分析.
- 在体外氨基化中的生物化学测试.
- 构建和测试仿真RNA分子.
主要成果:
- 鉴定了TLSTyr的5'域的明显的二次结构,与Bromoviridae的不同.
- 马病毒TLS的5'域对于有效的体外氨基化是必不可少的.
- 来自不同病毒家族的5'和3'域结合在一起的仿真TLSTyrRNA被成功氨基基化,表明功能模块化.
结论:
- 结构上独特的5'域的hordeivirusTLSTyr模仿的抗环,类似于它的Bromoviridae对应物.
- TLS轮胎元素表现出模块化,允许病毒家族之间的功能互换.
- 不同的TLSTyr结构为病毒RNA元素的进化及其与宿主因素的相互作用提供了洞察力.
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