机械化学重编程接口调节中性粒细胞杀菌活性和亡以预防植入物相关感染
Guangyu Chu1, Ming Guan1, Jiale Jin1
1Spine Lab, Department of Orthopedic Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310003, China.
Advanced materials (Deerfield Beach, Fla.)
|January 2, 2024
概括
这项研究设计了植入物表面 (CPZTs),通过增强中性粒细胞功能和促进骨愈合来预防感染. 这种新材料与细菌生物膜作斗争,并支持免疫反应,以更好地整合植入物.
科学领域:
- 生物材料科学 生物材料科学
- 免疫学 免疫学 免疫学
- 整形外科手术 整形外科手术
背景情况:
- 植入物相关感染 (IAI) 是一个主要的并发症,通常是由破坏中性粒细胞功能的细菌因素引起的.
- 植入后的细菌聚合和缺氧是IAI发展的关键风险因素.
研究的目的:
- 开发一种新型的植入物表面 (CPZTs),可以调节宿主免疫反应,以防止IAI.
- 增强中性粒细胞的功能,促进骨植入体的整合.
主要方法:
- 用 phytic 酸-Zn2+ 和 CaO2 纳米粒子 (CPZTs) 功能化的 TiO2 纳米柱阵列的制造.
- 评估CPZT抑制生物膜形成的能力,表现出抗菌粘附,机械杀菌和化学杀菌效应.
- 评估CPZTs对中性粒细胞功能,细胞亡和M2巨介导骨质生成 in vitro和 in vivo的影响.
主要成果:
- 工程 CPZTs 接口证明了新生生物膜形成的多性质抑制.
- 来自CPZT的持续氧化增强了中性粒细胞的活性氧物种 (ROS) 产生,以消除细菌.
- 表面调制加速了中性粒细胞的亡,并促进了M2巨细胞介导的骨质生成在试验室和老鼠IAI模型中.
结论:
- 通过免疫调节向中性粒细胞是预防IAI的可行策略.
- 开发出来的CPZTs表面有效地预防感染,并促进骨植入体的整合.
相关概念视频
01:30Bacterial Signaling
32.2K
Bacterial signaling can occur within bacteria (intracellular) or between bacteria (intercellular). At times, a group of bacteria behaves like a community. To achieve this, they engage in quorum sensing, the perception of higher cell density that causes changes in gene expression. Quorum sensing involves both extracellular and intracellular signaling. The signaling cascade starts with a molecule called an autoinducer (AI). Individual bacteria produce AIs that move out of the bacterial cell...
32.2K
01:28Cells of the Innate Immune Response
1.7K
The innate immune response is an immediate and non-specific response against pathogens, acting swiftly to prevent the spread of infections. The primary cells involved in this response are phagocytes and natural killer (NK) cells.
Phagocytes
Phagocytes police the peripheral tissues by removing cellular debris and responding to the invasion of foreign substances or pathogens. Many phagocytes attack and remove microorganisms even before lymphocytes detect them. The human body has two general...
Phagocytes
Phagocytes police the peripheral tissues by removing cellular debris and responding to the invasion of foreign substances or pathogens. Many phagocytes attack and remove microorganisms even before lymphocytes detect them. The human body has two general...
1.7K
01:18Surface Membrane Barriers
1.1K
The skin and mucous membranes serve as the primary line of defense against pathogens by providing both physical and chemical protection. These barriers are essential in preventing the entry and establishment of microbes, thereby maintaining the integrity of the host.
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...
1.1K
01:17Phagocytosis of Apoptotic Cells
3.8K
Cells undergoing apoptosis form apoptotic bodies that must be removed immediately to prevent inflammation, autoimmune diseases, and necrosis. Phagocytosis is carried out by professional phagocytes such as macrophages or immature dendritic cells. Non-professional phagocytes such as epithelial cells and fibroblasts also take part in this process; however, they are not as effective as professional phagocytes.
Normal cells contain receptors that prevent them from being recognized...
Normal cells contain receptors that prevent them from being recognized...
3.8K
01:31Defense Against Bacterial Pathogens
1.4K
The human immune system is a complex network of cells, tissues, and organs that work together to defend the body against bacterial infections. It consists of various immune cells, each playing a specific role in the defense mechanism.
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...
1.4K
01:23Antimicrobial Proteins
997
Antimicrobial proteins are important components of the immune system. They aid the body in combating pathogens by either killing them directly or hindering their replication processes. Four main types of antimicrobial substances are interferons, the complement system, iron-binding proteins, and antimicrobial proteins.
Interferons
Interferons (IFNs) are proteins produced by lymphocytes, macrophages, and fibroblasts infected with viruses. While IFNs cannot prevent viruses from entering and...
Interferons
Interferons (IFNs) are proteins produced by lymphocytes, macrophages, and fibroblasts infected with viruses. While IFNs cannot prevent viruses from entering and...
997