传染性人类诺罗病毒在河口水中的持久性
Daniel Rexin1, Andri T Rachmadi2, Joanne Hewitt2
1Institute of Environmental Science and Research Ltd. (ESR), Porirua, 5240, New Zealand. daniel.rexin@esr.cri.nz.
Food and environmental virology
|January 2, 2024
概括
诺罗病毒可以在河口水中保持至少3周的传染性,这对公众健康构成重大风险. 这项研究使用了一种新的培养方法来追踪不同温度下感染性诺罗病毒在水中的持久性.
科学领域:
- 环境微生物学环境微生物学
- 病毒学 病毒学
- 公共卫生 公共卫生
背景情况:
- 诺罗病毒是全球病毒性胃肠炎的主要原因.
- 污染的贝类是常见的诺罗病毒爆发的来源,与废水污染有关.
- 由于过去的培养限制,存在关于传染性诺罗病毒环境持久性的有限数据.
研究的目的:
- 为了确定传染性诺罗病毒在人造河口水中的环境持久性.
- 为了评估温度对诺罗病毒传染性随时间推移的影响.
- 调查诺罗病毒感染性和PCR检测之间的相关性.
主要方法:
- 利用一种新的人类肠道肠菌体培养方法进行诺罗病毒复制.
- 在黑暗中暴露在4°C和16°C的人造河口水 (25 ppt) 中的诺罗病毒,长达21天.
- 使用逆转录滴滴数字PCR (RT-ddPCR) 量化感染性诺罗病毒和测量病毒标位.
主要成果:
- 在整个21天的研究期间检测到传染性诺罗病毒.
- 到21日,相对感染率下降到3%,在16°C相比4°C的衰变率更高.
- 通过RT-ddPCR测量的诺罗病毒标位没有下降,突出显示了与传染性的脱节.
结论:
- 诺罗病毒在河口环境中至少在三周内仍然具有传染性.
- 温度会影响水中的诺罗病毒感染性衰变速度.
- 标准PCR检测方法可能无法准确地反映环境样本中诺罗病毒的传染潜力.
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