一种新型细菌菌体内素 (LysAB1245) 的表征,具有扩展的溶解活性,对与Acinetobacter baumannii耐药性相关的不同囊类型的抗药性
Rosesathorn Soontarach1,2, Potjanee Srimanote3, Buppa Arechanajan3
1Faculty of Science, Center of Antimicrobial Biomaterial Innovation-Southeast Asia, Prince of Songkla University, Songkhla, Thailand.
PloS one
|January 2, 2024
概括
一种新型的菌体内素,LysAB1245,显示出针对多药耐药的Acinetobacter baumannii的广泛抗菌活性. 这种内素有效降低了细菌的存活率,并显示出稳定性,这表明治疗医院感染的潜力.
科学领域:
- 微生物学 微生物学
- 分子生物学分子生物学
- 传染性疾病 传染性疾病
背景情况:
- 多抗药性宝曼尼菌 (MDR AB) 在医疗机构构成重大威胁,经常导致严重的医院感染.
- 囊中的多糖是MDR AB中关键的毒性因素,有助于其耐药性和致病性.
- 迫切需要新的治疗策略来对抗MDR AB感染.
研究的目的:
- 为了表达,净化和评估LysAB1245的抗菌活性,一个新型的菌体编码的endolysin.
- 评估LysAB1245对各种MDR囊型A. baumannii的疗效.
- 为了确定LysAB1245活动的稳定性和最佳条件.
主要方法:
- 从菌体T1245.5中表达和净化了LysAB1245.
- 对不同囊类型的临床MDR A. baumannii分离物和A. baumannii ATCC 19606的抗菌活性进行了测试.
- 确定了杀菌动力学,热稳定性,pH敏感性,最小抑制度 (MIC) 和最小杀菌度 (MBC).
主要成果:
- LysAB1245 对所有测试的MDR A. baumannii临床分离物和ATCC 19606具有广泛的活性.
- 用LysAB1245治疗导致细菌存活率在2小时内减少>3日志,并在30分钟内迅速杀菌活性.
- LysAB1245在4-70°C和广泛的pH范围 (4.5-10.5) 中表现出稳定性,MIC/MBC值为4.21μg/mL.
结论:
- LysAB1245 是一种强效的内溶酶,具有针对MDR A. baumannii的广泛杀菌活性.
- 它的稳定性和有效性表明,它具有作为医院MDR A. baumannii感染治疗剂的巨大潜力.
- 需要进一步研究LysAB1245作为一种治疗选择.
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