相关实验视频

Updated: Jul 6, 2025

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Hybrid De Novo Genome Assembly for the Generation of Complete Genomes of Urinary Bacteria using Short- and Long-read Sequencing Technologies

Published on: August 20, 2021

两种埃斯卡洛尼亚 (Escalloniales) 种的基因组组合草案

Andre S Chanderbali¹, Christopher Dervinis², Ioana G Anghel³

¹Florida Museum of Natural History, University of Florida, Gainesville, FL, USA. achander@ufl.edu.

BMC genomic data

|January 3, 2024

概括

本研究介绍了两个Escallonia物种的第一份基因组草案,为了解这种多样化的植物属的进化辐射及其在开花植物中的基因组定位提供了关键的基因组数据.

科学领域:

植物学植物学
基因组学就是基因组学.
进化生物学进化生物学

背景情况:

埃斯卡洛尼亚 (Escalloniaceae) 是埃斯卡洛尼亚科 (Escalloniales) 中的一个多样化属,这是一个在进化树上不确定的位置的植物类.
埃斯卡洛尼亚物种表现出显著的形态和生态变异,并且在新热带地区的生物多样性热点地区发现.

研究的目的:

为了阐明埃斯卡洛尼亚多样化的基因组基础.
为了澄清Escalloniales的遗传位置.
为比较进化基因组学产生有价值的数据.

主要方法:

通过牛津纳米孔和Illumina测序,为两个Escallonia物种 (E. rubra和E. herrerae) 制作了基因组草案.
基因组组装产生了566 Mb (E. rubra) 和994 Mb (E. herrerae) 的大小,并具有很高的连续性 (285 Kb和317 Kb的N50s).
基因组注释确定了31,038个 (E. rubra) 和47,905个 (E. herrerae) 蛋白质编码基因,具有很高的完整性 (约. 98%的组装,88%的注释).

主要成果:

为E. rubra和E. herrerae生成了高质量的基因组序列草案.
这些组合表现出良好的连续性和完整性,有超过31,000个和47,000个蛋白质编码基因被注释.
布斯科评估证实了高水平的基因组组装和注释完整性.

结论:

生成的基因组草案为未来对埃斯卡洛尼亚进化的研究提供了基础资源.
这些基因组数据将有助于理解埃斯卡洛尼亚的进化历史和遗传学关系.
该研究为新热带地区植物多样化的基因组基础提供了宝贵的见解.

关键词:

安第斯山脉安第斯山脉的安第斯山脉坎帕努利科群 (Campanulidae) 是一个群体.埃斯卡洛尼亚·赫雷雷 (Escallonia herrerae) 是一家在西班牙开放的酒店.埃斯卡洛尼亚红花 (Escallonia rubra) 是一种红花.基因组学就是基因组学.牛津纳米孔技术牛津纳米孔技术

更多相关视频

RNA-Seq Analysis of Differential Gene Expression in Electroporated Chick Embryonic Spinal Cord

RNA-Seq Analysis of Differential Gene Expression in Electroporated Chick Embryonic Spinal Cord

Published on: November 1, 2014

Novel Sequence Discovery by Subtractive Genomics

Novel Sequence Discovery by Subtractive Genomics

Published on: January 25, 2019

相关概念视频

Genome Annotation and Assembly

Genome Annotation and Assembly

The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.

Evolutionary Relationships through Genome Comparisons

Evolutionary Relationships through Genome Comparisons

Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...